Casas I, Powell L, Klapper P E, Cleator G M
Department of Pathological Sciences, Medical School, University of Manchester, UK.
J Virol Methods. 1995 May;53(1):25-36. doi: 10.1016/0166-0934(94)00173-e.
A new, rapid, and simple method for the isolation of either RNA or DNA from cerebrospinal fluid samples for subsequent amplification by specific polymerase chain reaction (PCR) assays is described. The technique involves a single extraction with a guanidinium thiocyanate acid (GuSCN) buffer, and does not require the use of organic solvents. Applied to the recovery of enteroviral RNA, herpes simplex virus (HSV) and Varicella-zoster virus (VZV) DNAs the method has proved to be of equivalent or better efficiency than established methods of nucleic acid separation but is less laborious and time consuming. The simplicity of the procedure permits the processing of large numbers of samples and the use of a single preparative method for either RNA or DNA PCR makes it an attractive method for the routine laboratory.
本文描述了一种从脑脊液样本中分离RNA或DNA的新方法，该方法快速、简便，可用于后续特定聚合酶链反应（PCR）检测的扩增。该技术采用硫氰酸胍（GuSCN）缓冲液进行单次提取，无需使用有机溶剂。应用于肠道病毒RNA、单纯疱疹病毒（HSV）和水痘带状疱疹病毒（VZV）DNA的回收时，该方法已被证明与已有的核酸分离方法效率相当或更高，但操作更简便、耗时更少。该程序的简单性允许处理大量样本，并且使用单一的制备方法进行RNA或DNA PCR，使其成为常规实验室的一种有吸引力的方法。
