Morey A L
Division of Anatomical Pathology, St Vincent's Hospital, Sydney, N.S.W., Australia.
J Pathol. 1995 Jun;176(2):113-21. doi: 10.1002/path.1711760204.
Non-isotopic in situ hybridization techniques are becoming increasingly widely used at the ultrastructural level, permitting rapid localization of nucleic acid targets with a high degree of resolution. Technical considerations dictate that the great specificity of the method cannot be matched by a similar degree of sensitivity; the value of non-isotopic ultrastructural in situ hybridization lies in its unique ability to localize nucleic acid targets in relation to submicroscopic cellular structures. This article presents an overview of non-isotopic ultrastructural hybridization methods and applications.
非同位素原位杂交技术在超微结构水平上的应用越来越广泛，能够以高分辨率快速定位核酸靶标。技术考量表明，该方法的高特异性无法与同等程度的灵敏度相匹配；非同位素超微结构原位杂交的价值在于其独特的能力，即能够相对于亚微观细胞结构定位核酸靶标。本文概述了非同位素超微结构杂交方法及其应用。
