高效的镁依赖性1型人类免疫缺陷病毒整合酶活性。
Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.
作者信息
Engelman A, Craigie R
机构信息
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-0560, USA.
出版信息
J Virol. 1995 Sep;69(9):5908-11. doi: 10.1128/JVI.69.9.5908-5911.1995.
Abstract
The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.
摘要
一般报道称，来自1型人类免疫缺陷病毒（HIV-1）的整合酶蛋白在体外有效发挥活性需要Mn2+。我们重新研究了HIV-1整合酶对二价金属离子的需求，发现该蛋白无论是在Mn2+还是Mg2+存在的情况下，均能够促进高效的3'加工和DNA链转移。金属离子的偏好取决于反应条件。与先前所述含Mn2+的反应混合物的反应条件相比，HIV-1整合酶在含Mg2+的反应混合物中表现出的非特异性核酸酶活性显著更低。
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