Sheng H Z, Lin P X, Nelson P G
Laboratory of Developmental Neurobiology, NICHD, NIH, Bethesda, MD 20892, USA.
Brain Res Mol Brain Res. 1995 Jun;30(2):196-202. doi: 10.1016/0169-328x(94)00291-l.
To address the question how relatively small numbers of immediate early gene (IEG) could specifically couple a wide range of stimulus-response cascades, we examined the possibility that IEG could be expressed heterogeneously in individual neurons. Analysis of multiple IEG in single neurons revealed that many individual DRG neurons express several IEGs. The combinatorial expression of IEGs by individual DRG displays substantial heterogeneity. Analysis of mRNA species encoding AP-1 composition in single cells also revealed coordinated change of mRNAs coding for AP-1 factors after membrane depolarization. Our results indicate that differential expression of IEG in individual cells, and the possible interaction among them may represent a mechanism by which the specificity in stimulation-response coupling may be achieved by IEGs.
为了解决相对少量的即刻早期基因（IEG）如何特异性地连接广泛的刺激-反应级联反应这一问题，我们研究了IEG在单个神经元中可能异质性表达的可能性。对单个神经元中的多种IEG进行分析发现，许多单个背根神经节（DRG）神经元表达多种IEG。单个DRG对IEG的组合表达显示出显著的异质性。对单细胞中编码AP-1组成的mRNA种类进行分析还发现，膜去极化后编码AP-1因子的mRNA会发生协同变化。我们的结果表明，单个细胞中IEG的差异表达以及它们之间可能的相互作用可能代表了一种机制，通过该机制IEG可以实现刺激-反应偶联的特异性。
