一株积累原卟啉IX的大肠杆菌突变体对蓝光的负趋光性。
Phototaxis away from blue light by an Escherichia coli mutant accumulating protoporphyrin IX.
作者信息
Yang H, Inokuchi H, Adler J
机构信息
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7332-6. doi: 10.1073/pnas.92.16.7332.
Abstract
The hemH gene of Escherichia coli encodes ferrochelatase (EC 4.99.1.1), the enzyme that catalyzes the last step in the production of heme, namely the synthesis of heme from protoporphyrin IX plus Fe2+. The behavioral responses to light were studied in E. coli carrying a hemH mutation. It was shown that the hemH mutant displayed a tumbling response upon illumination and a running response upon removal of the light. The most effect light to induce a tumbling response in the hemH mutant was blue light (396-450 nm). The chemotaxis machinery was needed for the light-induced tumbling response in the hemH mutant. The bacterial defect is an analog of the human inherited disease erythropoietic protoporphyria.
摘要
大肠杆菌的hemH基因编码铁螯合酶（EC 4.99.1.1），该酶催化血红素生成的最后一步，即由原卟啉IX和Fe2+合成血红素。对携带hemH突变的大肠杆菌的光行为反应进行了研究。结果表明，hemH突变体在光照时表现出翻滚反应，在去除光照后表现出奔跑反应。在hemH突变体中诱导翻滚反应最有效的光是蓝光（396 - 450纳米）。hemH突变体中光诱导的翻滚反应需要趋化机制。这种细菌缺陷类似于人类遗传性疾病红细胞生成性原卟啉症。
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