Tso W W, Adler J
J Bacteriol. 1974 May;118(2):560-76. doi: 10.1128/jb.118.2.560-576.1974.
Several methods for detecting or measuring negative chemotaxis are described. Using these, we have surveyed a number of chemicals for their ability to repel Escherichia coli. Although most of the repellents are harmful compounds, harmfulness is neither necessary nor sufficient to make a compound a repellent. The repellents can be grouped into at least nine classes according to (i) competition experiments, (ii) mutants lacking certain of the negative taxes, and (iii) their chemical structure. The specificity of each class was studied. It is suggested that each class corresponds to a distinct chemoreceptor. Generally, non-chemotactic mutants lack both positive and negative chemotaxis, and l-methionine is required for both kinds of taxis. Repellents at very low concentrations are not attractants, and attractants at very high concentrations are not repellents.
本文描述了几种检测或测量负趋化性的方法。利用这些方法，我们研究了多种化学物质对大肠杆菌的排斥能力。尽管大多数驱避剂都是有害化合物，但有害性既不是使一种化合物成为驱避剂的必要条件，也不是充分条件。根据（i）竞争实验、（ii）缺乏某些负趋化性的突变体以及（iii）它们的化学结构，这些驱避剂可至少分为九类。研究了每一类的特异性。结果表明，每一类都对应一种独特的化学感受器。一般来说，非趋化性突变体既缺乏正向趋化性也缺乏负向趋化性，两种趋化性都需要L-甲硫氨酸。极低浓度的驱避剂不是引诱剂，极高浓度的引诱剂也不是驱避剂。
