Karppinen J, Inkinen R I, Kääpä E, Lammi M J, Tammi M I, Holm S, Vanharanta H
Department of Physical Medicine and Rehabilitation, University of Oulu, Finland.
Spine (Phila Pa 1976). 1995 May 15;20(10):1170-7. doi: 10.1097/00007632-199505150-00012.
Eighteen pigs were stabbed with a scalpel in the anterior part of the anulus fibrosus of a lumbar disc. After surgery, the pigs received either tiaprofenic acid or indomethacin daily, and a third group did not receive any medication.
Nonsteroidal anti-inflammatory agents are widely used in the treatment of low back patients, but their long-term effects on the matrix molecules in the degenerate disc are unknown.
Several in vitro and in vivo studies on articular cartilage have suggested that tiaprofenic acid may not have adverse effects on matrix metabolism, whereas indomethacin probably does.
Uronic acid, DNA, and water contents were determined from five different locations in each injured disc. Transport and incorporation of sulfate were examined by in vivo radioactive tracer analysis, and proteoglycan structures were analyzed by gel electrophoresis.
Morphologically, there were no differences between the treatments. Tiaprofenic acid maintained a higher uronic acid content in the nucleus pulposus and outer anulus compared with that of the nonmedicated animals. Tiaprofenic acid decreased the incorporation of sulfate in the injured area and the water content at most sites. Indomethacin had no adverse effects compared with the nonmedicated group, and it increased water content in the posterior anulus fibrosus.
Long-term administration of tiaprofenic acid and indomethacin did not have harmful effects on matrix metabolism after disc injury. On the contrary, tiaprofenic acid may slightly protect proteoglycans in the degenerating disc.
用手术刀刺伤18头猪的腰椎间盘纤维环前部。术后，这些猪每日分别接受噻洛芬酸或吲哚美辛治疗，第三组不接受任何药物治疗。
非甾体抗炎药广泛用于治疗腰痛患者，但其对退变椎间盘基质分子的长期影响尚不清楚。
多项关于关节软骨的体外和体内研究表明，噻洛芬酸可能对基质代谢无不良影响，而吲哚美辛可能有。
测定每个损伤椎间盘中五个不同位置的糖醛酸、DNA和水含量。通过体内放射性示踪分析检测硫酸盐的转运和掺入情况，并通过凝胶电泳分析蛋白聚糖结构。
在形态学上，各治疗组之间无差异。与未用药的动物相比，噻洛芬酸使髓核和纤维环外层中的糖醛酸含量保持较高水平。噻洛芬酸降低了损伤区域硫酸盐的掺入以及大多数部位的水含量。与未用药组相比，吲哚美辛没有不良影响，且增加了纤维环后部的水含量。
椎间盘损伤后长期给予噻洛芬酸和吲哚美辛对基质代谢没有有害影响。相反，噻洛芬酸可能对退变椎间盘中的蛋白聚糖有轻微的保护作用。
