Kääpä E, Holm S, Inkinen R, Lammi M J, Tammi M, Vanharanta H
Department of Medical Chemistry, University of Helsinki, Finland.
J Spinal Disord. 1994 Aug;7(4):296-306.
An animal model of disk degeneration was used to study the concentration levels and types of proteoglycans in the different parts of the intervertebral disk. An annular incision was made with a scalpel blade into the anterior part of the porcine lumbar intervertebral disks via a retroperitoneal approach. Three months after injury the morphology of the injured disk had changed considerably. Disk height was diminished, and in the injured segment osteophytes had formed at the ventral edges of the vertebral body. The nucleus was small, fibrous, and yellowish. The annular lesion had healed by formation of granulation tissue, but the lamellar structure was partially destroyed. The concentration of inorganic [35S]sulfate had decreased across the whole disk, reflecting a decrease in the rate of solute transport. The concentration of incorporated [35S]sulfate had also decreased in the injured disks. The DNA concentration in the anterior annulus and in the nucleus had increased, whereas both the concentration of uronic acid and the ratio of chondroitin-6-sulfate to chondroitin-4-sulfate in the nucleus had decreased. Agarose gel electrophoresis combined with chondroitinase B digestion suggested the presence of dermatan sulfate proteoglycans in the injured annulus fibrosus. The morphology and chemical composition of the disks adjacent to the injured one were normal, and only a slight increase in the concentration of incorporated [35S]sulfate was observed in the disks above the injured one.
采用椎间盘退变动物模型研究椎间盘不同部位蛋白聚糖的浓度水平和类型。通过腹膜后入路，用手术刀在猪腰椎间盘前部做环形切口。损伤后三个月，损伤椎间盘的形态发生了显著变化。椎间盘高度减小，在损伤节段椎体前缘形成了骨赘。髓核小、纤维化且呈淡黄色。环形损伤通过肉芽组织形成而愈合，但板层结构部分被破坏。整个椎间盘内无机[35S]硫酸盐的浓度降低，反映溶质转运速率下降。损伤椎间盘内掺入的[35S]硫酸盐浓度也降低。前侧纤维环和髓核中的DNA浓度增加，而髓核中糖醛酸浓度以及硫酸软骨素-6-硫酸盐与硫酸软骨素-4-硫酸盐的比值均降低。琼脂糖凝胶电泳结合软骨素酶B消化表明，损伤的纤维环中存在硫酸皮肤素蛋白聚糖。与损伤椎间盘相邻的椎间盘形态和化学成分正常，仅在损伤椎间盘上方的椎间盘中观察到掺入的[35S]硫酸盐浓度略有增加。
