Jones T O, Whyte J N, Ginther N G, Townsend L D, Iwama G K
Department of Animal Science, Canadian Bacterial Diseases Network, University of British Columbia, Vancouver.
Toxicon. 1995 Mar;33(3):347-53. doi: 10.1016/0041-0101(94)00170-d.
The physiological effects of domoic acid on the immune system of marine invertebrates, which are known to accumulate this neurotoxin, have not been investigated previously. Changes in the number and relative phagocytic activity of circulating haemocytes in Crassostrea gigas exposed to domoic acid in the diatom Pseudonitzschia pungens f. multiseries for 48 hr, followed by clearance for 240 hr, were investigated using chemiluminescence. An initial stress response to the toxin assimilation was characterized by a marked increase in number and activity of haemocytes with 4 hr of exposure to the algae. Although the toxin level in the oyster increased during the 48 hr exposure, both number and activity of haemocytes declined from the 4 hr peak values to those significantly lower than control values after 24 hr clearance. This suppression in number and activity of circulating haemocytes following the initial toxin response was rectified only after 48 hr clearance, when domoic acid levels in the oyster tissue had declined to trace levels, allowing blood cells to regain their normal characteristics.
已知会积累这种神经毒素的软骨藻酸对海洋无脊椎动物免疫系统的生理影响，此前尚未得到研究。利用化学发光法，研究了暴露于含有多列拟菱形藻的硅藻中的太平洋牡蛎血细胞数量和相对吞噬活性的变化，暴露时间为48小时，随后清除240小时。对毒素同化的初始应激反应表现为，暴露于藻类4小时后，血细胞数量和活性显著增加。尽管在48小时的暴露期间牡蛎体内的毒素水平有所上升，但血细胞数量和活性在清除24小时后从4小时的峰值下降到显著低于对照值的水平。在最初的毒素反应后，循环血细胞数量和活性的这种抑制仅在清除48小时后得到纠正，此时牡蛎组织中的软骨藻酸水平已降至痕量水平，血细胞得以恢复其正常特征。
