Reifenberger G, Reifenberger J, Bilzer T, Wechsler W, Collins V P
Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany.
Am J Pathol. 1995 Aug;147(2):245-50.
The expression of epidermal growth factor receptor (EGFR) and the pre-pro form of one of its ligands, transforming growth factor-alpha (TGF-alpha), was studied by Northern blotting in a series of 14 capillary hemangioblastomas of the central nervous system. A constant coexpression of EGFR and pre-pro-TGF-alpha mRNAs was found. Immunocytochemical investigation of an extended series of 51 capillary hemangioblastomas revealed that the stromal cells in these tumors showed immunoreactivity with monoclonal antibodies to EGFR and TGF-alpha. Analysis of gene dosage by Southern blotting in 20 tumors indicated a normal gene copy number of EGFR and TGF alpha in all cases. Our findings suggest that autocrine and/or juxtacrine growth stimulation via the EGFR may contribute to tumor growth in capillary hemangioblastomas.
通过Northern印迹法对14例中枢神经系统毛细血管性血管母细胞瘤进行研究，检测表皮生长因子受体（EGFR）及其一种配体转化生长因子-α（TGF-α）前体形式的表达。发现EGFR和前体TGF-α mRNA持续共表达。对51例毛细血管性血管母细胞瘤进行扩大样本的免疫细胞化学研究显示，这些肿瘤中的基质细胞对EGFR和TGF-α单克隆抗体呈免疫反应性。通过Southern印迹法对20例肿瘤进行基因剂量分析表明，所有病例中EGFR和TGF-α的基因拷贝数均正常。我们的研究结果提示，通过EGFR的自分泌和/或旁分泌生长刺激可能促进毛细血管性血管母细胞瘤的肿瘤生长。
