Toyama Y, Kazama T, Fuse H, Katayama T
Department of Anatomy, School of Medicine, Chiba University, Japan.
Andrologia. 1995 May-Jun;27(3):165-70. doi: 10.1111/j.1439-0272.1995.tb01089.x.
A case of headless or decapitated spermatozoa of an infertile man is reported. The 33-year-old patient showed almost normal spermiogram except for a high percentage (more than 90%) of headless spermatozoa. Both hypoosmotic swelling and zona-free hamster egg tests showed that most headless spermatozoa appeared to be normal in their function as tails. The ultrastructure of the ejaculated spermatozoa was observed using a surface replica method and by conventional ultrathin sectioning. The headless spermatozoa were almost normal in structure except for the absence of the head. Tailless heads were rarely observed. Varieties of ultrastructural abnormalities were observed in spermatozoa heads. Among them, abnormalities in the nuclear membrane covering the posterior pole of the nucleus were prominent. The implantation fossa and the basal plate were not formed. Instead, the nuclear membrane with numerous nuclear pores was found in this region. The decapitation seemed to take place between the region of the basal plate and the proximal centriole.
本文报道了一例不育男性无头或断头精子的病例。该33岁患者的精液分析结果几乎正常，只是无头精子的比例很高（超过90%）。低渗肿胀试验和去透明带仓鼠卵试验均显示，大多数无头精子的尾部功能似乎正常。采用表面复型法和常规超薄切片法观察了射出精子的超微结构。无头精子除没有头部外，结构几乎正常。无尾的头部很少见。在精子头部观察到了多种超微结构异常。其中，覆盖细胞核后极的核膜异常突出。植入窝和基板未形成。相反，在该区域发现了有许多核孔的核膜。断头似乎发生在基板区域和近端中心粒之间。
