Baker C H, Matsuda S P, Liu D R, Corey E J
Department of Chemistry, Harvard University, Cambridge, MA 02138, USA.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):154-60. doi: 10.1006/bbrc.1995.2110.
Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predicted 83 kDa protein of 732 amino acids. The deduced amino acid sequence is 36-40% identical to known yeast and plant homologues and 83% identical to Rattus norvegicus lanosterol synthase. The new gene was shown to encode lanosterol synthase. The yeast lanosterol synthase deficient mutant SMY8 was complemented by the human gene, and a cell-free homogenate of SMY8 expressing the human gene was shown to convert 2,3-oxidosqualene to lanosterol.
羊毛甾醇合酶[（S）-2,3-环氧角鲨烯变位酶（环化，形成羊毛甾醇），EC 5.4.99.7]在形成甾醇核的反应中催化（S）-2,3-氧化角鲨烯环化生成羊毛甾醇。我们在此报告编码羊毛甾醇合酶的人类基因（OSC）的克隆和特性分析，该酶是一种预测的由732个氨基酸组成的83 kDa蛋白质。推导的氨基酸序列与已知的酵母和植物同源物有36%-40%的同一性，与褐家鼠羊毛甾醇合酶有83%的同一性。新基因被证明编码羊毛甾醇合酶。缺乏羊毛甾醇合酶的酵母突变体SMY8被人类基因互补，并且表达人类基因的SMY8的无细胞匀浆被证明能将2,3-氧化角鲨烯转化为羊毛甾醇。
