Manicourt D H, Fujimoto N, Obata K, Thonar E J
Saint Luc University Hospital, University of Louvain, Brussels, Belgium.
Arthritis Rheum. 1995 Aug;38(8):1031-9. doi: 10.1002/art.1780380803.
To measure serum levels of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of MMP-1 (TIMP-1) in patients with rheumatoid arthritis (RA) and in age-matched control subjects, and to determine how these correlate with serum levels of antigenic keratan sulfate (KS) and other biochemical and clinical indicators of disease activity.
Immunoassays were used to measure levels of MMP-1, MMP-3, TIMP-1, and antigenic KS. Radiologic and functional joint scores were based upon Steinbrocker's criteria. Erythrocyte sedimentation rates (ESR) and levels of C-reactive proteins (CRP) were measured.
In RA patients, levels of MMP-3 and TIMP-1 were significantly increased, and strongly correlated with the ESR and CRP levels but not with radiologic or functional joint scores. Levels of antigenic KS were significantly lower in RA patients and correlated negatively with systemic parameters of inflammation and serum levels of TIMP-1.
The increase in serum levels of MMP-3 and TIMP-1 appears to reflect systemic inflammation in RA. The inverse correlation between serum levels of TIMP-1 and antigenic KS suggests that an upregulation of TIMP-1 synthesis might be responsible for the apparent suppression of cartilage aggrecan catabolism in patients with severe inflammatory changes.
测定类风湿关节炎（RA）患者及年龄匹配的对照者血清中基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和MMP-1组织抑制剂（TIMP-1）的水平，并确定这些指标与抗原性硫酸角质素（KS）的血清水平以及疾病活动的其他生化和临床指标之间的相关性。
采用免疫测定法测定MMP-1、MMP-3、TIMP-1和抗原性KS的水平。放射学和关节功能评分基于斯坦布鲁克标准。测定红细胞沉降率（ESR）和C反应蛋白（CRP）水平。
在RA患者中，MMP-3和TIMP-1水平显著升高，且与ESR和CRP水平密切相关，但与放射学或关节功能评分无关。RA患者的抗原性KS水平显著降低，且与全身炎症参数和TIMP-1血清水平呈负相关。
血清MMP-3和TIMP-1水平升高似乎反映了RA中的全身炎症。TIMP-1血清水平与抗原性KS之间的负相关表明，TIMP-1合成上调可能是严重炎症改变患者软骨聚集蛋白聚糖分解代谢明显受抑制的原因。
