Matton D P, Mau S L, Okamoto S, Clarke A E, Newbigin E
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Plant Mol Biol. 1995 Aug;28(5):847-58. doi: 10.1007/BF00042070.
Genomic clones encoding the S2- and S6-RNases of Nicotiana alata Link and Otto, which are the allelic stylar products of the self-incompatibility (S) locus, were isolated and sequenced. Analysis of genomic DNA by pulsed-field gel electrophoresis and Southern blotting indicates the presence of only a single S-RNase gene in the N. alata genome. The sequences of the open-reading frames in the genomic and corresponding cDNA clones were identical. The organization of the genes was similar to that of other S-RNase genes from solanaceous plants. No sequence similarity was found between the DNA flanking the S2- and S6-RNase genes, despite extensive similarities between the coding regions. The DNA flanking the S6-RNase gene contained sequences that were moderately abundant in the genome. These repeat sequences are also present in other members of the Nicotianae.
编码烟草（Nicotiana alata Link and Otto）S2和S6核糖核酸酶的基因组克隆已被分离和测序，这两种核糖核酸酶是自交不亲和性（S）位点的等位花柱产物。通过脉冲场凝胶电泳和Southern印迹对基因组DNA进行分析，结果表明烟草基因组中仅存在一个S - 核糖核酸酶基因。基因组和相应cDNA克隆中开放阅读框的序列是相同的。这些基因的结构与茄科植物中其他S - 核糖核酸酶基因的结构相似。尽管编码区之间存在广泛相似性，但在S2和S6核糖核酸酶基因侧翼的DNA之间未发现序列相似性。S6核糖核酸酶基因侧翼的DNA包含在基因组中中度丰富的序列。这些重复序列也存在于烟草属的其他成员中。
