Helariutta Y, Elomaa P, Kotilainen M, Griesbach R J, Schröder J, Teeri T H
Institute of Biotechnology, University of Helsinki, Finland.
Plant Mol Biol. 1995 Apr;28(1):47-60. doi: 10.1007/BF00042037.
Recent studies on chalcone synthase (CHS) and the related stilbene synthase (STS) suggest that the structure of chs-like genes in plants has evolved into different forms, whose members have both different regulation and capacity to code for different but related enzymatic activities. We have studied the diversity of chs-like genes by analysing the structure, expression patterns and catalytic properties of the corresponding enzymes of three genes that are active during corolla development in Gerbera hybrida. The expression patterns demonstrate that chs-like genes are representatives of three distinct genetic programmes that are active during organ differentiation in gerbera. Gchs1 and gchs3 code for typical CHS enzymes, and their gene expression pattern temporally correlates with flavonol (gchs1, gchs3) and anthocyanin (gchs1) synthesis during corolla development. Gchs2 is different. The expression pattern does not correlate with the pigmentation pattern, the amino acid sequence deviates considerably from the consensus of typical CHSs, and the catalytic properties are different. The data indicate that it represents a new member in the large superfamily of chs and chs-related genes.
最近关于查尔酮合酶（CHS）及相关芪合酶（STS）的研究表明，植物中chs样基因的结构已演变成不同形式，其成员在调控方面以及编码不同但相关酶活性的能力方面均存在差异。我们通过分析三种在杂种非洲菊（Gerbera hybrida）花冠发育过程中具有活性的基因所对应的酶的结构、表达模式及催化特性，研究了chs样基因的多样性。表达模式表明，chs样基因是非洲菊器官分化过程中活跃的三种不同遗传程序的代表。Gchs1和gchs3编码典型的CHS酶，它们的基因表达模式在时间上与花冠发育过程中的黄酮醇（gchs1、gchs3）和花青素（gchs1）合成相关。Gchs2则不同。其表达模式与色素沉着模式不相关，氨基酸序列与典型CHS的共有序列有很大差异，且催化特性也不同。数据表明，它代表了chs和chs相关基因大家族中的一个新成员。
