Hernández I, Chacin J
Laboratorio de Investigaciones Gastrointestinales, Facultad de Medicina, Universidad del Zulia, Venezuela.
Acta Physiol Pharmacol Ther Latinoam. 1994;44(1-2):43-7.
The effects of steroids on gastric acid secretion have been studied in different animal species with contradictory results. In the present work we investigated direct effects of hydrocortisone on acid secretion in the isolated toad gastric mucosa (Bufo marinus). The rate of hydrogen ion secretion (QH+) was measured using the pH-stat method of Durbin and Heinz. Hydrocortisone significantly stimulated QH+ in a dose dependent manner. The effect tended to reach a maximum with the concentration of 10mM and lasted for at least one hour. In the mucosa pre-treated with 1mM cimetidine, an specific antagonist of histamine H2 receptors, the addition of 10mM hydrocortisone did not stimulate QH+, but addition of the cholinergic agonist carbachol did produce a significant stimulation in the rate of acid secretion. In the gastric mucosa previously stimulated with 5mM hydrocortisone, the addition of a submaximal dose of histamine increased QH+ and this effect was greater than that provoked by histamine in the absence of the steroid. The results indicate that hydrocortisone significantly stimulates acid secretion in a dose-dependent manner in the isolated toad gastric mucosa. This effect seems to be dependent on the activation of H2 receptors.
人们在不同动物物种中研究了类固醇对胃酸分泌的影响，结果相互矛盾。在本研究中，我们调查了氢化可的松对离体蟾蜍胃黏膜（海蟾蜍）胃酸分泌的直接影响。采用德宾和海因茨的pH稳态法测量氢离子分泌速率（QH+）。氢化可的松以剂量依赖的方式显著刺激QH+。在浓度为10mM时，这种作用趋于达到最大值，并持续至少一小时。在用1mM西咪替丁（一种组胺H2受体特异性拮抗剂）预处理的黏膜中，添加10mM氢化可的松不会刺激QH+，但添加胆碱能激动剂卡巴胆碱确实会显著刺激胃酸分泌速率。在先前用5mM氢化可的松刺激的胃黏膜中，添加亚最大剂量的组胺会增加QH+，且这种作用大于在无类固醇情况下组胺引起的作用。结果表明，氢化可的松在离体蟾蜍胃黏膜中以剂量依赖的方式显著刺激胃酸分泌。这种作用似乎依赖于H2受体的激活。
