Lemic-Stojcevic L, Nias A H, Breathnach A S
Richard Dimbleby Department of Cancer Research, UMDS, St. Thomas' Hospital Campus, London, U.K.
Exp Dermatol. 1995 Apr;4(2):79-81. doi: 10.1111/j.1600-0625.1995.tb00226.x.
Using a clonogenic assay in vitro, it has been shown that exposure to azelaic acid (1-100 mM) for 24 hours has a dose-dependent effect on the survival of the colony-forming ability of murine (B16) and human (HMB2, and SK23) melanoma cells as compared with a non-melanotic non-tumoral Chinese hamster cell line (CHO). Both human cell lines were more sensitive to the diacid than the murine cells, and the HMB2 cells were more sensitive than the SK23 cells. These differences may be partly correlated with differences in pigmentation and doubling times between the three melanoma cell lines. The two human lines were more pigmented than the B16, and the SK23 more than the HMB2; the human lines had a longer doubling time than the others.
体外克隆形成试验表明，与非黑素性非肿瘤中国仓鼠细胞系（CHO）相比，将壬二酸（1 - 100 mM）作用24小时，对鼠源（B16）和人源（HMB2及SK23）黑色素瘤细胞集落形成能力的存活具有剂量依赖性影响。两种人源细胞系对该二酸的敏感性均高于鼠源细胞，且HMB2细胞比SK23细胞更敏感。这些差异可能部分与三种黑色素瘤细胞系在色素沉着和倍增时间上的差异相关。两种人源细胞系的色素沉着程度高于B16，且SK23高于HMB2；人源细胞系的倍增时间比其他细胞系更长。
