A sliver-gold impregnation technique for routine neuropathological use.

A silver-gold impregnation technique for routine neuropathological use is presented. It is easy to perform, gives reproducible results and the ingredients are chemically well characterized. It has been successfully applied to formalin-fixed paraffin-embedded material from human brain, where nerve cell processes, neurofibrillary changes and melanin-containing neurons are distinctly visualized. Axons in peripheral nerves are impregnated and cross striations in muscle cells can also be demonstrated.