Feigin I, Naoumenko J
J Neuropathol Exp Neurol. 1976 Sep-Oct;35(5):495-507. doi: 10.1097/00005072-197609000-00002.
Some of the chemical principles involved in some silver and gold staining procedures for neuropathological studies are analyzed in the light of experience with methods for astrocytes, microglia, axons and reticulin fibers. The role of formalin fixation, and of treatment with ammonium bromide or oxidizing agents prior to staining for glial cells or reticulin fibers respectively, is described. The silver staining methods are divided into two fundamentally different groups. One is based on the use of silver diammine solutions with or without other substances such as carbonates or pyridine, and at varying times, gold toning. This group encompasses a great variety of techniques for staining glial cells, reticulin fibers and axons. The second group is based on the use of weak solutions of silver ions, reduction in a photographic developer, treatment with gold chloride, and a second reduction in oxalic acid. This group is represented by methods for staining axons and reticulin fibers. A brief analysis of the gold and mercuric chloride methods for astrocytes is also given.
根据对星形胶质细胞、小胶质细胞、轴突和网状纤维染色方法的经验，分析了一些用于神经病理学研究的银染和金染程序中涉及的化学原理。描述了福尔马林固定的作用，以及分别在对神经胶质细胞或网状纤维染色之前用溴化铵或氧化剂处理的作用。银染方法基本上分为两个不同的组。一组基于使用含或不含其他物质（如碳酸盐或吡啶）的二氨银溶液，并在不同时间进行金调色。这一组包括用于染色神经胶质细胞、网状纤维和轴突的多种技术。第二组基于使用弱银离子溶液，在显影剂中还原，用氯化金处理，以及在草酸中再次还原。这一组以轴突和网状纤维染色方法为代表。还简要分析了星形胶质细胞的金染和氯化汞染色方法。
