Suppr超能文献

变形链球菌细胞表面蛋白抗原I/II的T细胞、黏附及B细胞表位

T-cell, adhesion, and B-cell epitopes of the cell surface Streptococcus mutans protein antigen I/II.

作者信息

Kelly C G, Todryk S, Kendal H L, Munro G H, Lehner T

机构信息

Department of Immunology, United Medical School at Guy's Hospital, London, United Kingdom.

出版信息

Infect Immun. 1995 Sep;63(9):3649-58. doi: 10.1128/iai.63.9.3649-3658.1995.

Abstract

The T-cell and antibody responses to a cell surface streptococcal antigen (SA I/II) were investigated in naturally sensitized humans. Serum antibody responses were directed predominantly to the N-terminal (residues 39 to 481) and central (residues 816 to 1213) regions of SA I/II which may be involved in bacterial adhesion to salivary receptors. T-cell responses were also directed predominantly towards the central region. The linear peptide relationship of the immunodominant and minor T- and B-cell as well as adhesion epitopes was mapped within residues 816 to 1213. Immunodominant T-cell and B-cell epitopes were identified within residues 803 to 853, which were separated in linear sequence from the adhesion epitopes (residues 1005 to 1044). Adhesion epitopes overlapped with minor B- and T-cell epitopes (residues 1005 to 1054 and 1085 to 1134). An immunodominant promiscuous T-cell epitope (residues 985 to 1004) was adjacent to an adhesion epitope (residues 1005 to 1024). The limited B-cell response to adhesion epitopes is consistent with the success of Streptococcus mutans in colonizing the oral cavity. The strategy of T-cell, adhesion, and B-cell epitope mapping has revealed a general approach for identifying components of subunit vaccines which may focus responses to critical functional determinants. Such epitopes of SA I/II may constitute the components of a subunit vaccine against dental caries.

摘要

在自然致敏的人体中研究了对细胞表面链球菌抗原(SA I/II)的T细胞和抗体反应。血清抗体反应主要针对SA I/II的N端(第39至481位氨基酸残基)和中央区域(第816至1213位氨基酸残基),这些区域可能参与细菌与唾液受体的黏附。T细胞反应也主要针对中央区域。在第816至1213位氨基酸残基范围内绘制了免疫显性和次要T细胞、B细胞以及黏附表位的线性肽关系图。在第803至853位氨基酸残基内鉴定出免疫显性T细胞和B细胞表位,它们在序列上与黏附表位(第1005至1044位氨基酸残基)分开。黏附表位与次要B细胞和T细胞表位重叠(第1005至1054位氨基酸残基和第1085至1134位氨基酸残基)。一个免疫显性的通用性T细胞表位(第985至1004位氨基酸残基)与一个黏附表位(第1005至1024位氨基酸残基)相邻。对黏附表位的有限B细胞反应与变形链球菌在口腔中定植成功是一致的。T细胞、黏附及B细胞表位图谱绘制策略揭示了一种识别亚单位疫苗成分的通用方法,该方法可能使反应聚焦于关键功能决定因素。SA I/II的此类表位可能构成抗龋齿亚单位疫苗的成分。

相似文献

1
T-cell, adhesion, and B-cell epitopes of the cell surface Streptococcus mutans protein antigen I/II.
Infect Immun. 1995 Sep;63(9):3649-58. doi: 10.1128/iai.63.9.3649-3658.1995.
6
Identification of a repeated epitope recognized by human serum antibodies in a surface protein antigen of Streptococcus mutans.
Oral Microbiol Immunol. 1996 Apr;11(2):121-8. doi: 10.1111/j.1399-302x.1996.tb00346.x.
7
A protein fragment of streptococcal cell surface antigen I/II which prevents adhesion of Streptococcus mutans.
Infect Immun. 1993 Nov;61(11):4590-8. doi: 10.1128/iai.61.11.4590-4598.1993.
10

引用本文的文献

1
Glycosylation of oral bacteria in modulating adhesion and biofilm formation.
J Oral Microbiol. 2025 Apr 8;17(1):2486650. doi: 10.1080/20002297.2025.2486650. eCollection 2025.
2
The Multifaceted Nature of Streptococcal Antigen I/II Proteins in Colonization and Disease Pathogenesis.
Front Microbiol. 2020 Nov 25;11:602305. doi: 10.3389/fmicb.2020.602305. eCollection 2020.
4
Total IgA and IgA reactivity to antigen I/II epitopes in HLA-DRB1*04 positive subjects.
Open J Immunol. 2013 Sep 1;3(3):82-92. doi: 10.4236/oji.2013.33012.
5
Alterations in immunodominance of Streptococcus mutans AgI/II: lessons learned from immunomodulatory antibodies.
Vaccine. 2014 Jan 9;32(3):375-82. doi: 10.1016/j.vaccine.2013.11.023. Epub 2013 Nov 16.
6
Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation.
Mol Microbiol. 2011 Aug;81(4):1034-49. doi: 10.1111/j.1365-2958.2011.07749.x. Epub 2011 Jul 15.
8
Deleted in malignant brain tumors-1 protein (DMBT1): a pattern recognition receptor with multiple binding sites.
Int J Mol Sci. 2010;11(12):5212-33. doi: 10.3390/ijms1112521. Epub 2010 Dec 17.
9
Pooled protein immunization for identification of cell surface antigens in Streptococcus sanguinis.
PLoS One. 2010 Jul 26;5(7):e11666. doi: 10.1371/journal.pone.0011666.
10
The changing faces of Streptococcus antigen I/II polypeptide family adhesins.
Mol Microbiol. 2010 Jul;77(2):276-86. doi: 10.1111/j.1365-2958.2010.07212.x. Epub 2010 May 24.

本文引用的文献

1
Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans.
Infect Immun. 1993 Apr;61(4):1547-52. doi: 10.1128/iai.61.4.1547-1552.1993.
2
Promiscuous and allele-specific anchors in HLA-DR-binding peptides.
Cell. 1993 Jul 16;74(1):197-203. doi: 10.1016/0092-8674(93)90306-b.
3
The structure and function of proline-rich regions in proteins.
Biochem J. 1994 Jan 15;297 ( Pt 2)(Pt 2):249-60. doi: 10.1042/bj2970249.
5
High-affinity binding of short peptides to major histocompatibility complex class II molecules by anchor combinations.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4456-60. doi: 10.1073/pnas.91.10.4456.
6
Calcium-binding properties of SSP-5, the Streptococcus gordonii M5 receptor for salivary agglutinin.
Infect Immun. 1994 Dec;62(12):5220-6. doi: 10.1128/iai.62.12.5220-5226.1994.
7
A protein fragment of streptococcal cell surface antigen I/II which prevents adhesion of Streptococcus mutans.
Infect Immun. 1993 Nov;61(11):4590-8. doi: 10.1128/iai.61.11.4590-4598.1993.
8
Suppressive effect of antibody on processing of T cell epitopes.
J Exp Med. 1993 Oct 1;178(4):1459-63. doi: 10.1084/jem.178.4.1459.
10
Identification of antigenic epitopes in a surface protein antigen of Streptococcus mutans in humans.
Infect Immun. 1994 Sep;62(9):4034-42. doi: 10.1128/iai.62.9.4034-4042.1994.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验