Kelly C G, Todryk S, Kendal H L, Munro G H, Lehner T
Department of Immunology, United Medical School at Guy's Hospital, London, United Kingdom.
Infect Immun. 1995 Sep;63(9):3649-58. doi: 10.1128/iai.63.9.3649-3658.1995.
The T-cell and antibody responses to a cell surface streptococcal antigen (SA I/II) were investigated in naturally sensitized humans. Serum antibody responses were directed predominantly to the N-terminal (residues 39 to 481) and central (residues 816 to 1213) regions of SA I/II which may be involved in bacterial adhesion to salivary receptors. T-cell responses were also directed predominantly towards the central region. The linear peptide relationship of the immunodominant and minor T- and B-cell as well as adhesion epitopes was mapped within residues 816 to 1213. Immunodominant T-cell and B-cell epitopes were identified within residues 803 to 853, which were separated in linear sequence from the adhesion epitopes (residues 1005 to 1044). Adhesion epitopes overlapped with minor B- and T-cell epitopes (residues 1005 to 1054 and 1085 to 1134). An immunodominant promiscuous T-cell epitope (residues 985 to 1004) was adjacent to an adhesion epitope (residues 1005 to 1024). The limited B-cell response to adhesion epitopes is consistent with the success of Streptococcus mutans in colonizing the oral cavity. The strategy of T-cell, adhesion, and B-cell epitope mapping has revealed a general approach for identifying components of subunit vaccines which may focus responses to critical functional determinants. Such epitopes of SA I/II may constitute the components of a subunit vaccine against dental caries.
在自然致敏的人体中研究了对细胞表面链球菌抗原(SA I/II)的T细胞和抗体反应。血清抗体反应主要针对SA I/II的N端(第39至481位氨基酸残基)和中央区域(第816至1213位氨基酸残基),这些区域可能参与细菌与唾液受体的黏附。T细胞反应也主要针对中央区域。在第816至1213位氨基酸残基范围内绘制了免疫显性和次要T细胞、B细胞以及黏附表位的线性肽关系图。在第803至853位氨基酸残基内鉴定出免疫显性T细胞和B细胞表位,它们在序列上与黏附表位(第1005至1044位氨基酸残基)分开。黏附表位与次要B细胞和T细胞表位重叠(第1005至1054位氨基酸残基和第1085至1134位氨基酸残基)。一个免疫显性的通用性T细胞表位(第985至1004位氨基酸残基)与一个黏附表位(第1005至1024位氨基酸残基)相邻。对黏附表位的有限B细胞反应与变形链球菌在口腔中定植成功是一致的。T细胞、黏附及B细胞表位图谱绘制策略揭示了一种识别亚单位疫苗成分的通用方法,该方法可能使反应聚焦于关键功能决定因素。SA I/II的此类表位可能构成抗龋齿亚单位疫苗的成分。