Todryk S M, Kelly C G, Munro G H, Lehner T
Department of Immunology, United Medical and Dental Schools of Guy's & St Thomas's, London, UK.
Immunology. 1996 Jan;87(1):55-63.
Antibodies directed against the cell surface adhesin, termed streptococcal antigen I/II of Streptococcus mutans can protect against dental caries. Streptococcal antigen I/II (SA I/II) interacts with salivary glycoproteins and promotes adhesion to the tooth surface. Topical application of monoclonal antibodies which recognize a domain within residues 816-1213 (fragment 3) prevents colonization by S. mutans in primates. In this study the immunogenicity and antigenicity of fragment 3 was investigated in five strains of mice. Fragment 3 induced an immune response following immunization with whole cells of S. mutans in all strains of mice. Immunization with recombinant fragment 3 also induced T-cell proliferative and antibody responses both to fragment 3 and to the SA I/II. Antibody responses to the previously defined adhesion determinants (residues 1005-1044) were weak or undetectable. Immunization of three representative strains of mice with a recombinant polypeptide (residues 975-1044) comprising this adhesion epitope and an adjacent T-cell epitope (residues 975-1004) elicited both T- and B-cell responses to the polypeptide and to native SA I/II. The B-cell epitopes overlapped with the adhesion determinant. These findings provide a means of directing immune responses to functional determinants of SA I/II.
针对变形链球菌细胞表面粘附素（称为链球菌抗原I/II）的抗体可预防龋齿。链球菌抗原I/II（SA I/II）与唾液糖蛋白相互作用并促进对牙齿表面的粘附。局部应用识别816 - 1213位残基内一个结构域（片段3）的单克隆抗体可防止变形链球菌在灵长类动物中定植。在本研究中，对五株小鼠的片段3的免疫原性和抗原性进行了研究。在所有小鼠品系中，用变形链球菌全细胞免疫后，片段3诱导了免疫反应。用重组片段3免疫也诱导了针对片段3和SA I/II的T细胞增殖和抗体反应。对先前确定的粘附决定簇（1005 - 1044位残基）的抗体反应较弱或无法检测到。用包含该粘附表位和相邻T细胞表位（975 - 1004位残基）的重组多肽（975 - 1044位残基）免疫三株代表性小鼠品系，引发了针对该多肽和天然SA I/II的T细胞和B细胞反应。B细胞表位与粘附决定簇重叠。这些发现提供了一种针对SA I/II功能决定簇引导免疫反应的方法。
