参与天蓝色链霉菌A3(2)相变可变phi C31抗性机制的两个基因。
Two genes involved in the phase-variable phi C31 resistance mechanism of Streptomyces coelicolor A3(2).
作者信息
Bedford D J, Laity C, Buttner M J
机构信息
Department of Genetics, John Innes Centre, Colney, Norwich, United Kingdom.
出版信息
J Bacteriol. 1995 Aug;177(16):4681-9. doi: 10.1128/jb.177.16.4681-4689.1995.
Abstract
The phage growth limitation (Pgl) system of Streptomyces coelicolor confers resistance to phi C31 and its homoimmune phages. The positions of the pgl genes within a 16-kb clone of S. coelicolor DNA were defined by subcloning, insertional inactivation, and deletion mapping. Nucleotide sequencing and functional analysis identified two genes, pglY and pglZ, required for the Pgl+ (phage-resistant) phenotype. pglY and pglZ, which may be translationally coupled, are predicted to encode proteins with M(r)S of 141,000 and 104,000, respectively. Neither protein shows significant similarity to other known proteins, but PglY has a putative ATP/GTP binding motif. The pglY and pglZ genes are cotranscribed from a single promoter which appears to be constitutive and is not induced by phage infection.
摘要
天蓝色链霉菌的噬菌体生长限制（Pgl）系统赋予对phi C31及其同免疫噬菌体的抗性。通过亚克隆、插入失活和缺失定位确定了天蓝色链霉菌DNA 16 kb克隆内pgl基因的位置。核苷酸测序和功能分析鉴定出Pgl +（噬菌体抗性）表型所需的两个基因pglY和pglZ。pglY和pglZ可能是翻译偶联的，预计分别编码分子量为141,000和104,000的蛋白质。这两种蛋白质与其他已知蛋白质均无明显相似性，但PglY具有一个假定的ATP / GTP结合基序。pglY和pglZ基因从一个单一启动子共转录，该启动子似乎是组成型的，不受噬菌体感染诱导。
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