• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

An alleged yeast polyphosphate kinase is actually diadenosine-5', 5"'-P1,P4-tetraphosphate alpha,beta-phosphorylase.

作者信息

Booth J W, Guidotti G

机构信息

Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

出版信息

J Biol Chem. 1995 Aug 18;270(33):19377-82. doi: 10.1074/jbc.270.33.19377.

DOI:10.1074/jbc.270.33.19377
PMID:7642617
Abstract

Polyphosphates are a major constituent of the yeast Saccharomyces cerevisiae. A purification of the enzyme polyphosphate kinase (E.C. 2.7.4.1) from this organism has been reported (Felter, S., and Stahl, A.J.C. (1973) Biochimie (Paris) 55, 245-251). The assay for activity used in this purification was the production of 32P-labeled nucleotide, presumed to be ATP, in the presence of [32P]polyphosphate and ADP. We have found that this assay does not reflect the activity of a polyphosphate kinase but rather the combination of an exopolyphosphatase, releasing free [32P]phosphate from the added [32P]polyphosphate, and the ADP-[32P]phosphate exchange activity of the enzyme diadenosine 5',5"'-P1, P4-tetraphosphate alpha, beta-phosphorylase (Ap4A phosphorylase). We also present direct evidence for the formation of an enzyme-AMP intermediate in the actin of Ap4A phosphorylase.

摘要

相似文献

1
An alleged yeast polyphosphate kinase is actually diadenosine-5', 5"'-P1,P4-tetraphosphate alpha,beta-phosphorylase.
J Biol Chem. 1995 Aug 18;270(33):19377-82. doi: 10.1074/jbc.270.33.19377.
2
Phosphorolytic cleavage of diadenosine 5',5'''-P1,P4-tetraphosphate. Properties of homogeneous diadenosine 5',5'''-P1,P4-tetraphosphate alpha, beta-phosphorylase from Saccharomyces cerevisiae.5',5'''-P1,P4-四磷酸二腺苷的磷酸解裂解。来自酿酒酵母的均一5',5'''-P1,P4-四磷酸二腺苷α,β-磷酸化酶的性质。
J Biol Chem. 1985 Mar 25;260(6):3542-7.
3
Diadenosine 5',5'''-P1, P4-tetraphosphate alpha, beta-phosphorylase from yeast supports nucleoside diphosphate-phosphate exchange.来自酵母的二腺苷5′,5‴-P1,P4-四磷酸α,β-磷酸化酶可支持核苷二磷酸-磷酸交换。
J Biol Chem. 1986 May 5;261(13):5943-6.
4
Chromate, molybdate, tungstate and vanadate behave as substrates of yeast diadenosine 5',5'''-p1,p4-tetraphosphate alpha, beta-phosphorylase.铬酸盐、钼酸盐、钨酸盐和钒酸盐可作为酵母二腺苷5',5'''-p1,p4-四磷酸α,β-磷酸化酶的底物。
Biochimie. 1986 May;68(5):757-60. doi: 10.1016/s0300-9084(86)80170-5.
5
Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase.萤火虫荧光素酶催化的二核苷多磷酸的合成。
Eur J Biochem. 1991 Dec 5;202(2):507-13. doi: 10.1111/j.1432-1033.1991.tb16402.x.
6
Yeast diadenosine 5',5'''-P1,P4-tetraphosphate alpha,beta-phosphorylase behaves as a dinucleoside tetraphosphate synthetase.酵母二腺苷5′,5″′-P1,P4-四磷酸α,β-磷酸化酶表现为二核苷四磷酸合成酶。
Biochemistry. 1987 Jul 28;26(15):4763-8. doi: 10.1021/bi00389a025.
7
Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) phosphorylase activity.水华鱼腥藻和其他蓝细菌具有二腺苷5′,5″-P1,P4-四磷酸（Ap4A）磷酸化酶活性。
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):795-800. doi: 10.1042/bj3200795.
8
Structures of yeast Apa2 reveal catalytic insights into a canonical AP₄A phosphorylase of the histidine triad superfamily.酵母 Apa2 的结构揭示了组氨酸三联体超家族中典型的 AP₄A 磷酸化酶的催化见解。
J Mol Biol. 2013 Aug 9;425(15):2687-98. doi: 10.1016/j.jmb.2013.04.018. Epub 2013 Apr 26.
9
Immunoaffinity chromatography of diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase from Saccharomyces cerevisiae.
Biotechnol Appl Biochem. 1990 Jun;12(3):276-83.
10
Chemical modification of a functional arginine residue in diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase I from Saccharomyces cerevisiae.酿酒酵母中二腺苷5',5'''-P1,P4-四磷酸（Ap4A）磷酸化酶I中功能性精氨酸残基的化学修饰。
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):135-9. doi: 10.1042/bj2790135.

引用本文的文献

1
Structure and mechanism of an ADP-glucose phosphorylase from Arabidopsis thaliana.拟南芥ADP - 葡萄糖磷酸化酶的结构与机制
Biochemistry. 2006 Mar 14;45(10):3154-62. doi: 10.1021/bi052232m.
2
Enhanced phosphate uptake and polyphosphate accumulation in Burkholderia cepacia grown under low pH conditions.在低pH条件下生长的洋葱伯克霍尔德菌中增强的磷酸盐摄取和多聚磷酸盐积累。
Microb Ecol. 2002 Jul;44(1):69-77. doi: 10.1007/s00248-002-3004-x. Epub 2002 Apr 4.
3
Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases.
Hint、Fhit和GalT：核苷酸水解酶和转移酶的组氨酸三联体超家族三个分支的功能、结构、进化及作用机制
Biochemistry. 2002 Jul 23;41(29):9003-14. doi: 10.1021/bi025942q.
4
Role of polyphosphate kinase in biofilm formation by Porphyromonas gingivalis.多聚磷酸激酶在牙龈卟啉单胞菌生物膜形成中的作用
Infect Immun. 2002 Aug;70(8):4708-15. doi: 10.1128/IAI.70.8.4708-4715.2002.
5
Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH.酵母Humicola念珠菌G-1在酸性pH条件下对多磷酸盐的细胞内积累。
Appl Environ Microbiol. 2000 Sep;66(9):4068-73. doi: 10.1128/AEM.66.9.4068-4073.2000.
6
Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins.HINT的晶体结构表明，组氨酸三联体蛋白是与半乳糖基转移酶相关的核苷酸结合蛋白。
Nat Struct Biol. 1997 Mar;4(3):231-8. doi: 10.1038/nsb0397-231.
7
Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) phosphorylase activity.水华鱼腥藻和其他蓝细菌具有二腺苷5′,5″-P1,P4-四磷酸（Ap4A）磷酸化酶活性。
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):795-800. doi: 10.1042/bj3200795.