强啡肽A增强小鼠腹腔巨噬细胞的吞噬作用。

Enhancement of phagocytosis by dynorphin A in mouse peritoneal macrophages.

作者信息

Ichinose M, Asai M, Sawada M

机构信息

Department of Physiology, Shimane Medical University, Izumo, Japan.

出版信息

J Neuroimmunol. 1995 Jul;60(1-2):37-43. doi: 10.1016/0165-5728(95)00050-c.

DOI:10.1016/0165-5728(95)00050-c

PMID:7642746

Abstract

The effects of the opioid peptide dynorphin A (DynA) on phagocytosis in peritoneal macrophages was examined by flow cytometry (FCM). DynA enhanced phagocytosis in a dose-dependent manner. Leucine-enkephalin (Leu-Enk), methionine-enkephalin (Met-Enk), beta-neo-endorphin (beta Neo-End), DynA(9-17) and DynA(13-17) had no such activity. Alpha-Neo-endorphin (alpha Neo-End), dynorphin B (DynB), DynA(1-13) and DynA(6-17) enhanced phagocytosis less effectively than DynA. Naloxone did not inhibit the enhancement of phagocytosis induced by DynA. Unstimulated control phagocytosis was partially suppressed in Ca2+-free EGTA-containing solution and even in this solution DynA enhanced phagocytosis. However, the enhancement by DynA was suppressed in EGTA- and BAPTA-AM-containing Ca2+-free solution. The present study showed that enhancement of phagocytosis by DynA was independent of extracellular Ca2+ ([Ca2+]o) and dependent on intracellular Ca2+ ([Ca2+]i). The present results support DynA being one of the mediators from the nervous system that modulates the immune system.

摘要

采用流式细胞术（FCM）检测阿片肽强啡肽A（DynA）对腹腔巨噬细胞吞噬作用的影响。DynA以剂量依赖方式增强吞噬作用。亮氨酸脑啡肽（Leu-Enk）、甲硫氨酸脑啡肽（Met-Enk）、β-新内啡肽（βNeo-End）、DynA(9 - 17)和DynA(13 - 17)无此活性。α-新内啡肽（αNeo-End）、强啡肽B（DynB）、DynA(1 - 13)和DynA(6 - 17)增强吞噬作用的效果不如DynA。纳洛酮不抑制DynA诱导的吞噬作用增强。在不含Ca2+的含EGTA溶液中，未刺激的对照吞噬作用部分受到抑制，即使在此溶液中DynA仍能增强吞噬作用。然而，在含EGTA和BAPTA-AM的无Ca2+溶液中，DynA的增强作用受到抑制。本研究表明，DynA增强吞噬作用与细胞外Ca2+（[Ca2+]o）无关，而与细胞内Ca2+（[Ca2+]i）有关。本研究结果支持DynA是调节免疫系统的神经系统介质之一。