酸性成纤维细胞生长因子（aFGF）片段增强小鼠腹腔巨噬细胞的吞噬作用。

Enhancement of phagocytosis in mouse peritoneal macrophages by fragments of acidic fibroblast growth factor (aFGF).

作者信息

Ichinose M, Sawada M, Sasaki K, Oomura Y

机构信息

Department of Physiology, Shimane Medical University, Izumo, Japan.

出版信息

Int J Immunopharmacol. 1998 Apr-May;20(4-5):193-204. doi: 10.1016/s0192-0561(98)00028-9.

DOI:10.1016/s0192-0561(98)00028-9

PMID:9730255

Abstract

To characterize the effects of acidic fibroblast growth factor (aFGF) in mouse peritoneal macrophages, the effects of aFGF fragments on phagocytosis were examined. Fragments that were tested included aFGF(1-15), aFGF(1-20), aFGF(1-29), Ala16-aFGF(1-29), aFGF(9-29) and aFGF(114-140). aFGF(1-29) induced an enhancement of phagocytosis in a dose-dependent manner and was more effective than any other fragments tested. Even in Ca2+-and Mg2+-free solutions, phagocytosis was enhanced by aFGF(1-29). However, the enhancement induced by aFGF(1-29) was completely inhibited in the presence of mannan (4 mg/ml). Furthermore, the enhancement of phagocytosis by aFGF(1-29) was suppressed by heparin (100 microg/ml). The results of the present study suggest that the active region of aFGF that is responsible for the enhancement of phagocytosis corresponds to residues 15-29 and that phagocytosis, which is modulated by aFGF, is independent of extracellular Ca2+ and is mediated by mannose receptors.

摘要

为了表征酸性成纤维细胞生长因子（aFGF）对小鼠腹腔巨噬细胞的影响，研究了aFGF片段对吞噬作用的影响。所测试的片段包括aFGF(1 - 15)、aFGF(1 - 20)、aFGF(1 - 29)、Ala16 - aFGF(1 - 29)、aFGF(9 - 29)和aFGF(114 - 140)。aFGF(1 - 29)以剂量依赖方式诱导吞噬作用增强，且比所测试的任何其他片段都更有效。即使在无Ca2 +和Mg2 +的溶液中，aFGF(1 - 29)也能增强吞噬作用。然而，在存在甘露聚糖（4 mg/ml）的情况下，aFGF(1 - 29)诱导的增强作用被完全抑制。此外，肝素（100 μg/ml）可抑制aFGF(1 - 29)对吞噬作用的增强。本研究结果表明，aFGF中负责增强吞噬作用的活性区域对应于第15 - 29位氨基酸残基，并且aFGF调节的吞噬作用独立于细胞外Ca2 +，由甘露糖受体介导。