Hennig B, Toborek M, Boissonneault G A, Shantha N C, Decker E A, Oeltgen P R
Department of Nutrition and Food Science, University of Kentucky, Lexington 40506, USA.
J Nutr. 1995 Aug;125(8):2045-54. doi: 10.1093/jn/125.8.2045.
Enrichment of lipoproteins with fatty acids derived from animal and/or plant fats may modify the oxidizability of lipoproteins and their effects on endothelial barrier function. To test this hypothesis, rabbits were fed for 30 days diets containing 2 g corn oil/100 g diet (low fat diet) or low fat supplemented with 16 g/100 g diet of corn oil, corn oil with added cholesterol, milk fat, chicken fat, beef tallow or lard. Compared with those fed the low fat, serum and LDL cholesterol concentrations were significantly lower in rabbits fed corn oil and greater in animals fed corn oil with added cholesterol or chicken fat. In contrast to the cholesterol data, lipid hydroperoxide levels were highest in oxidized LDL derived from rabbits fed corn oil or lard. LDL vitamin E levels were highest in rabbits fed corn oil with added cholesterol. The significant elevations in linoleic acid [18:2(n-6)] in serum and LDL may partially explain the high oxidizability of LDL in rabbits fed corn oil. LDL isolated from animals fed corn oil, lard or milk fat had significantly greater albumin transfer across cultured endothelial monolayers compared with those of the low fat diet group. Their oxidative modification further contributed to endothelial barrier dysfunction. Dietary cholesterol supplementation to the corn oil diet decreased oxidizability of LDL and partially protected the oxidized LDL-mediated endothelial cell dysfunction as compared with the corn oil diet group. These data suggest that beef tallow and chicken fat are the least atherogenic fats if oxidative modification of LDL is a critical issue in atherosclerosis.
用来自动物和/或植物脂肪的脂肪酸富集脂蛋白可能会改变脂蛋白的氧化能力及其对内皮屏障功能的影响。为了验证这一假设，给兔子喂食30天含有2克玉米油/100克日粮的低脂日粮，或低脂日粮中添加16克/100克日粮的玉米油、添加胆固醇的玉米油、乳脂、鸡脂肪、牛脂或猪油。与喂食低脂日粮的兔子相比，喂食玉米油的兔子血清和低密度脂蛋白胆固醇浓度显著降低，而喂食添加胆固醇的玉米油或鸡脂肪的动物血清和低密度脂蛋白胆固醇浓度更高。与胆固醇数据相反，来自喂食玉米油或猪油的兔子的氧化低密度脂蛋白中的脂质过氧化氢水平最高。喂食添加胆固醇的玉米油的兔子的低密度脂蛋白维生素E水平最高。血清和低密度脂蛋白中亚油酸[18:2(n-6)]的显著升高可能部分解释了喂食玉米油的兔子中低密度脂蛋白的高氧化能力。与低脂日粮组相比，从喂食玉米油、猪油或乳脂的动物中分离出的低密度脂蛋白在培养的内皮单层上的白蛋白转运显著更高。它们的氧化修饰进一步导致内皮屏障功能障碍。与玉米油日粮组相比，在玉米油日粮中添加膳食胆固醇可降低低密度脂蛋白的氧化能力，并部分保护氧化低密度脂蛋白介导的内皮细胞功能障碍。这些数据表明，如果低密度脂蛋白的氧化修饰是动脉粥样硬化的关键问题，牛脂和鸡脂肪是最不易致动脉粥样硬化的脂肪。
