Toborek M, Feldman D L, Hennig B
Department of Surgery, University of Kentucky Medical Center, Lexington 40536, USA.
J Am Coll Nutr. 1997 Feb;16(1):32-8. doi: 10.1080/07315724.1997.10718646.
To test the hypothesis that dietary fats, depending on the fat source, may modulate aortic lipid peroxidation and antioxidant protection.
Rabbits were fed a low fat (LF, 2 g/100 g corn oil) diet or LF enriched with 16 g/100 g (w/w) of corn oil (CO), corn oil plus cholesterol (23.5 mg/100 g diet, CO + C), bovine milk fat (MF), chicken fat (CF), beef tallow (BT) or lard (L). After a 30-day feeding period, aortic lipid peroxidation, as well as antioxidant enzymes and vitamin E were measured.
In rabbits fed CO or L, aortic TBARS (a marker of lipid peroxidation) and total glutathione concentrations were greater but vitamin E levels were lower compared with the LF treatment. Moreover, in rabbits fed CO, elevated activities of glutathione peroxidase and glutathione reductase but lowered activity of superoxide dismutase were observed. In rabbits fed the remaining high fat diets, including the CO + C diet, aortic lipid peroxidation and antioxidant activities/levels did not differ from those fed LF. Feeding rabbits high-fat diets for 30 days did not induce aortic lipid deposition.
The present results indicate CO, and possibly L, as the fat sources which significantly increase aortic oxidative stress. Because long-term disturbances in redox status may be implicated in atherogenesis, excessive dietary intake of CO or L may significantly contribute to the injury of the vessel wall.
检验膳食脂肪根据脂肪来源可能调节主动脉脂质过氧化和抗氧化保护作用这一假设。
给兔子喂食低脂（LF，2克/100克玉米油）饮食或添加16克/100克（w/w）玉米油（CO）、玉米油加胆固醇（23.5毫克/100克饮食，CO + C）、牛乳脂肪（MF）、鸡脂肪（CF）、牛脂（BT）或猪油（L）的低脂饮食。经过30天的喂养期后，测量主动脉脂质过氧化以及抗氧化酶和维生素E。
与LF处理相比，喂食CO或L的兔子主动脉丙二醛（脂质过氧化的标志物）和总谷胱甘肽浓度更高，但维生素E水平更低。此外，喂食CO的兔子中，谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性升高，但超氧化物歧化酶的活性降低。喂食包括CO + C饮食在内的其余高脂肪饮食的兔子，主动脉脂质过氧化和抗氧化活性/水平与喂食LF的兔子没有差异。给兔子喂食30天高脂肪饮食未诱导主动脉脂质沉积。
目前的结果表明，CO以及可能的L是显著增加主动脉氧化应激的脂肪来源。由于氧化还原状态的长期紊乱可能与动脉粥样硬化的发生有关，过量摄入CO或L可能会显著导致血管壁损伤。
