Wang X, Wang W, Bengmark S, Andersson R
Department of Surgery, Lund University Hospital, Sweden.
J Surg Res. 1995 Sep;59(3):326-36. doi: 10.1006/jsre.1995.1172.
Potential alterations in lipid metabolism in the early stage of acute liver failure are poorly elucidated. In the present study, acute liver failure was induced by subtotal hepatectomy (90%) in the rat in order to investigate early alterations in lipid contents in blood, hepatocytes, and enterocytes. Hypocholesterolemia and hypotriglyceridemia appeared 2 and 6 hr following subtotal hepatectomy. Plasma levels of high density lipoprotein-cholesterol were significantly lower in rats with acute liver failure than in controls, which may be associated with hypocholesterolemia. An increase in erythrocyte phospholipids and triglycerides was seen from 2 hr on after hepatectomy. The content of phospholipids and triglycerides was reduced in isolated enterocytes from the proximal small intestine and increased in enterocytes from the distal small intestine. Isolated hepatocytes from the remnant liver exhibited an increase in phosphatidylethanolamine and a decrease in phosphatidylinositol. Levels of enterocyte phosphatidylserine were elevated in both the proximal and the distal small intestine, while they diminished in the distal small intestine. Sphingomyelin content increased in the proximal small intestine. The recognition of lipid alterations in the intestine-liver-systemic circulation axis in the early stage of acute liver failure may be beneficial in improving the recovery from acute liver failure.
急性肝衰竭早期脂质代谢的潜在变化尚未得到充分阐明。在本研究中，通过大鼠肝大部切除术（90%）诱导急性肝衰竭，以研究血液、肝细胞和肠细胞中脂质含量的早期变化。肝大部切除术后2小时和6小时出现低胆固醇血症和低甘油三酯血症。急性肝衰竭大鼠的血浆高密度脂蛋白胆固醇水平显著低于对照组，这可能与低胆固醇血症有关。肝切除术后2小时起红细胞磷脂和甘油三酯增加。近端小肠分离的肠细胞中磷脂和甘油三酯含量降低，而远端小肠的肠细胞中含量增加。残余肝脏分离的肝细胞中磷脂酰乙醇胺增加，磷脂酰肌醇减少。近端和远端小肠肠细胞的磷脂酰丝氨酸水平均升高，而在远端小肠中则降低。近端小肠中鞘磷脂含量增加。认识急性肝衰竭早期肠-肝-体循环轴中的脂质变化可能有助于改善急性肝衰竭的恢复。
