Widerström L, Hamberg K, Bratthall D
Department of Cariology, Centre of Oral Health Sciences, University of Lund, Sweden.
Oral Microbiol Immunol. 1995 Feb;10(1):26-34. doi: 10.1111/j.1399-302x.1995.tb00114.x.
Analysis of the salivary immunoglobulin A (IgA) antibody activity to antigens separated from oral streptococci was carried out on 49 individuals in 11 families. The aim of this study were to i) study the human salivary IgA activity within families to antigens separated from reference strains of Streptococcus mutans, Streptococcus sobrinus and Streptococcus parasanguis and ii) to search for potential differences in the salivary IgA activity to the streptococcal antigens several years apart. The immunoblot revealed similarity in saliva IgA response within the families. A majority of the bands, approximately 7 (median) for each extract was found in both child and parent. A few bands (approximately 3) were found in parents but not in children. Only one to two bands were found in children's saliva without a corresponding band in any of the parents. The antibody activity of saliva samples obtained several years apart was essentially unchanged, especially considering the total number of bands. Differences in the relative intensity of the bands could be seen, and a few bands appeared only in some immunoblots for a specific subject. For 3 of 4 children participating in the longitudinal study, more bands against mutans streptococci appeared with age.
对11个家庭的49名个体进行了唾液免疫球蛋白A（IgA）针对从口腔链球菌分离出的抗原的抗体活性分析。本研究的目的是：i）研究家庭内人类唾液IgA对变形链球菌、远缘链球菌和血链球菌参考菌株分离出的抗原的活性；ii）探寻相隔数年的唾液IgA对链球菌抗原的活性的潜在差异。免疫印迹显示家庭内唾液IgA反应具有相似性。在儿童和父母的唾液中均发现了大部分条带，每种提取物约有7条（中位数）。在父母唾液中发现了少数条带（约3条），但在儿童唾液中未发现。在儿童唾液中仅发现一两条条带，而在任何一位父母的唾液中均无相应条带。相隔数年采集的唾液样本的抗体活性基本未变，尤其是考虑到条带总数时。条带相对强度存在差异，并且一些条带仅在特定个体的某些免疫印迹中出现。参与纵向研究的4名儿童中有3名，随着年龄增长，针对变形链球菌的条带增多。
