Widerström L, Bratthall D, Hamberg K
Department of Cariology, Faculty of Odontology, University of Lund, Sweden.
Oral Microbiol Immunol. 1994 Oct;9(5):278-83. doi: 10.1111/j.1399-302x.1994.tb00071.x.
The aims of this study were i) to characterize and compare the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein patterns of reference Streptococcus mutans and Streptococcus sobrinus strains, subcultivated for years, with fresh isolates of the same serotype; ii) to study possible differences between the human salivary immunoglobulin A (IgA) activity to reference strains and to fresh bacterial isolates of the saliva donors; iii) to examine for potential differences in the salivary IgA activity to the streptococcal antigens during 1 week; and iv) to map, in the same individuals, the serum IgA activity against the selected bacteria. S. mutans reference and fresh isolated strains showed a similar protein pattern with few exceptions. The immunoblot also revealed similarity in saliva IgA response, with only one subject's saliva displaying clearly one band's difference. For S. sobrinus a larger discrepancy was seen. The antibody activity during the one week interval was essentially unchanged. When incubated with serum, a different immunoblot profile was seen compared with saliva, although most bands revealed by saliva were also displayed by serum.
i）对多年传代培养的变形链球菌和远缘链球菌参考菌株与相同血清型的新鲜分离株进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳蛋白质图谱分析并比较；ii）研究人类唾液免疫球蛋白A（IgA）对参考菌株和唾液供体新鲜细菌分离株的活性差异；iii）检测1周内唾液中针对链球菌抗原的IgA活性的潜在差异；iv）在同一受试者中绘制血清针对所选细菌的IgA活性图谱。变形链球菌参考菌株和新鲜分离株显示出相似的蛋白质图谱，仅有少数例外。免疫印迹还显示唾液IgA反应相似，只有一名受试者的唾液显示出明显的一条带的差异。对于远缘链球菌，差异更大。一周内抗体活性基本不变。与血清孵育时，与唾液相比出现了不同的免疫印迹图谱，尽管唾液显示的大多数条带血清中也有。
