Nicolas-Bolnet C, Dieterlen-Lievre F
Institut d'Embryologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Collège de France, Nogent s/Marne.
Poult Sci. 1995 Jun;74(6):942-50. doi: 10.3382/ps.0740942.
Growth of hemopoietic cells from chick embryonic aorta, spleen, and bone marrow was obtained in a serum-free culture medium containing 10-d-old chick embryo extract. When cells were cultured in semi-solid medium (plasma clot), they proliferated and differentiated to generate colonies committed to the granuloid or erythroid lineage. Mixed colonies composed either of granulocytes and macrophages or granulocytes and erythroblasts were also obtained. In addition, two different types of colonies composed of immature cells (blast cells) could be distinguished by the size of the cells. These colonies were composed of larger cells originating from the more immature progenitors, because they developed after a longer incubation period. Growth of bone marrow cells up to 3 wk in liquid culture was also obtained in the serum-free culture supplemented with chick embryo extract. These cells retained a very immature phenotype and the ability to differentiate when reseeded in an appropriate medium. These results indicate for the first time that chick embryo extracts contain hematopoietic growth factors that are active in differentiation and proliferation of chick hematopoietic cells.
在含有10日龄鸡胚提取物的无血清培养基中，实现了鸡胚胎主动脉、脾脏和骨髓造血细胞的生长。当细胞在半固体培养基（血浆凝块）中培养时，它们会增殖并分化，形成粒细胞或红细胞系的集落。还获得了由粒细胞和巨噬细胞或粒细胞和成红细胞组成的混合集落。此外，由未成熟细胞（原始细胞）组成的两种不同类型的集落可以通过细胞大小来区分。这些集落由源自更不成熟祖细胞的较大细胞组成，因为它们在更长的孵育期后形成。在补充了鸡胚提取物的无血清培养中，骨髓细胞在液体培养中也能生长长达3周。这些细胞保留了非常不成熟的表型，并且在接种到合适的培养基中时具有分化能力。这些结果首次表明，鸡胚提取物含有对鸡造血细胞的分化和增殖有活性的造血生长因子。
