Yun T K, Kim S H, Lee Y S
Laboratory of Cancer Pathology, Korea Cancer Center Hospital, Seoul.
Anticancer Res. 1995 May-Jun;15(3):839-45.
A new medium-term in vivo model was tried using pulmonary adenoma induced by benzo(a)pyrene (BP) in newborn mice. Both inbred mice such as C57BL/5J, C57BR/cdJ. A/J mice and non inbred N:GP(S) mice were used. Benzo(a)pyrene was injected in the subscapular region of newborn mice within 24 hours after birth at a dose of 0.5 mg and 1 mg per mouse, respectively. After 9 weeks lung tumor induced in N:GP(S) and A/J mice but in the other mice. The dose showing a 50% tumor incidence was found in N:GP(S) mice to be 0.5 mg of BP but the tumor incidence was very high in A/J mice even at 40 micrograms of BP, the lowest dose in this experiment. To verify the utility of this model, ascorbic acid, carrot, beta carotene, soybean lecithin, spinach, Sesamum indicum, Ganoderma lucidum, caffeine, red ginseng extract, fresh ginseng and 13-cis retinoic acid, some of which are known to have anticarcinogenic activity in various animal models, were tried with this system. Ascorbic acid, soybean lecithin, Ganoderma lucidum, caffeine and red ginseng extract showed inhibition of lung tumor incidence, while fresh ginseng, carrot, beta carotene, spinach and 13-cis retinoic acid did not. This result suggested that the 9-week medium-term model using lung tumor induced by 0.5 mg of BP was useful for the screening of cancer preventive agents.
尝试使用新生小鼠中由苯并(a)芘（BP）诱导的肺腺瘤建立一种新的中期体内模型。使用了近交系小鼠，如C57BL/5J、C57BR/cdJ、A/J小鼠以及非近交系N:GP(S)小鼠。在出生后24小时内，分别以每只小鼠0.5毫克和1毫克的剂量将苯并(a)芘注射到新生小鼠的肩胛下区域。9周后，N:GP(S)和A/J小鼠诱导出肺肿瘤，而其他小鼠未出现。发现N:GP(S)小鼠中导致50%肿瘤发生率的剂量为0.5毫克BP，但在A/J小鼠中，即使是本实验中最低剂量40微克的BP，肿瘤发生率也非常高。为了验证该模型的实用性，在此系统中尝试使用了抗坏血酸、胡萝卜、β-胡萝卜素、大豆卵磷脂、菠菜、芝麻、灵芝、咖啡因、红参提取物、鲜人参和13-顺式维甲酸，其中一些在各种动物模型中已知具有抗癌活性。抗坏血酸、大豆卵磷脂、灵芝、咖啡因和红参提取物显示出对肺肿瘤发生率的抑制作用，而鲜人参、胡萝卜、β-胡萝卜素、菠菜和13-顺式维甲酸则没有。该结果表明，使用0.5毫克BP诱导肺肿瘤的9周中期模型可用于筛选癌症预防剂。
