Berkova N, Pagé M
Laboratoire d'Endocrinologie de la Reproduction, Hospital St-François d'Assise, Québec, Canada.
Anticancer Res. 1995 May-Jun;15(3):863-6.
Some reports indicate that the cytotoxic activity of hTNFa and taxol might be enhanced when combined with certain chemotherapeutic agents. To assess if taxol might modulate the action of hTNF alpha, we have studied the cytotoxic action of hTNF alpha in combination with taxol on two ovarian carcinoma cell lines, the SKOV3 and SKVLB. These two cell lines are resistant to the action of hTNF alpha. The SKVLB cells overexpressed the multidrug resistant gene (MDR-1) which confers a resistance to taxol. We observed that the combination of low concentration of hTNF alpha with taxol could increase the cytotoxic activity of taxol in SKOV3 cells. Furthermore, a four-hour pretreatment with taxol before adding hTNF alpha to the culture has shown a significant increase in their cytotoxic activity. This increase was not observed at the same level in SKOV3 cells pretreated with hTNF alpha. However, the phenomena was not observed in SKVLB cells. This indicates that taxol could alter the mechanism of hTNF alpha-resistance.
一些报告表明，当与某些化疗药物联合使用时，人肿瘤坏死因子α（hTNFα）和紫杉醇的细胞毒性活性可能会增强。为了评估紫杉醇是否可能调节hTNFα的作用，我们研究了hTNFα与紫杉醇联合对两种卵巢癌细胞系SKOV3和SKVLB的细胞毒性作用。这两种细胞系对hTNFα的作用具有抗性。SKVLB细胞过表达多药耐药基因（MDR - 1），该基因赋予对紫杉醇的抗性。我们观察到低浓度的hTNFα与紫杉醇联合可增加紫杉醇对SKOV3细胞的细胞毒性活性。此外，在向培养物中添加hTNFα之前用紫杉醇预处理4小时已显示其细胞毒性活性显著增加。在用hTNFα预处理的SKOV3细胞中未观察到相同程度的这种增加。然而，在SKVLB细胞中未观察到该现象。这表明紫杉醇可以改变hTNFα抗性的机制。
