桑萎蔫病菌梨火疫病菌和栎叶焦枯病菌致病型的随机扩增多态性DNA分析
Randomly amplified polymorphic DNA analysis of Xylella fastidiosa Pierce's disease and oak leaf scorch pathotypes.
作者信息
Chen J, Lamikanra O, Chang C J, Hopkins D L
机构信息
Center for Viticultural Science, Florida A&M University, Tallahassee 32307, USA.
出版信息
Appl Environ Microbiol. 1995 May;61(5):1688-90. doi: 10.1128/aem.61.5.1688-1690.1995.
Abstract
Randomly amplified polymorphic DNA analysis was conducted with 14 primers to 17 strains of Xylella fastidiosa. There was a high degree of similarity among the seven Pierce's disease (PD) strains (Sxy > 0.93) and the seven oak leaf scorch (OLS) strains (Sxy > 0.96). However, the two groups were different, with a similarity index of 0.67, confirming the presence of a PD DNA cluster and suggesting the presence of a new OLS cluster. The control plum leaf scald strains (two strains) together with the periwinkle wilt strain had a much smaller similarity index (0.44) compared with the PD and OLS clusters.
摘要
利用14种引物对17株桑萎蔫病菌进行了随机扩增多态性DNA分析。7株梨火疫病（PD）菌株（Sxy > 0.93）和7株栎叶焦枯病（OLS）菌株（Sxy > 0.96）之间具有高度相似性。然而，这两组菌株有所不同，相似性指数为0.67，证实存在一个PD DNA簇，并表明存在一个新的OLS簇。对照李叶焦枯病菌株（两株）与长春花枯萎病菌株相比，与PD和OLS簇的相似性指数要小得多（0.44）。
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