Lawrence L M, Harvey J, Gilmour A
Department of Food Science (Food Microbiology), Queen's University of Belfast, Northern Ireland, United Kingdom.
Appl Environ Microbiol. 1993 Sep;59(9):3117-9. doi: 10.1128/aem.59.9.3117-3119.1993.
The 10-mer primer OPM-01 (5'-GTT GGT GGC T-3') was used to generate random amplification of polymorphic DNA (RAPD) profiles by polymerase chain reaction for 91 strains of Listeria monocytogenes from raw milk, food, and veterinary, medical, and food-environmental sources. The profiles obtained contained 1 to 10 bands within the molecular size range of 0.5 to 5.0 kbp. Reproducibility was enhanced by annealing at low stringency and introducing a 1-min ramp time between annealing and extension temperatures. Thirty-three RAPD profiles were observed, with specific profiles being observed for strains from each source. RAPD profiles allowed discrimination within serogroups, although five RAPD profiles which were not confined to one serotype were found. Within food strains, one RAPD profile was more common than others, suggesting this to be a common type among strains from this source.
使用10聚体引物OPM - 01（5'-GTT GGT GGC T-3'）通过聚合酶链反应对来自生牛奶、食品以及兽医、医疗和食品环境来源的91株单核细胞增生李斯特菌进行随机扩增多态性DNA（RAPD）分析。获得的图谱在0.5至5.0 kbp的分子大小范围内包含1至10条带。通过低严谨度退火以及在退火温度和延伸温度之间引入1分钟的升温时间提高了重复性。观察到33种RAPD图谱，每种来源的菌株都有特定的图谱。尽管发现了5种不限于一种血清型的RAPD图谱，但RAPD图谱可在血清群内进行区分。在食品菌株中，一种RAPD图谱比其他图谱更常见，表明这是该来源菌株中的常见类型。
