在表达木糖异构酶基因（xyl1）的重组酿酒酵母中，以葡萄糖作为共底物进行厌氧木糖转化过程中的木糖醇形成及还原当量生成
Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the xyl1 gene.
作者信息
Thestrup H N, Hahn-Hägerdal B
机构信息
Applied Microbiology, Lund Institute of Technology, Lund University, Sweden.
出版信息
Appl Environ Microbiol. 1995 May;61(5):2043-5. doi: 10.1128/aem.61.5.2043-2045.1995.
Abstract
Glucose was used as a cosubstrate under anaerobic conditions in the conversion of xylose to xylitol by a recombinant Saccharomyces cerevisiae strain expressing the xyl1 gene. Glucose was metabolized mainly through glycolysis, with carbon dioxide, acetate, and ethanol as end products and with reduction equivalents generated in the glyceraldehyde-3-phosphate dehydrogenase and acetaldehyde dehydrogenase reactions. At a high glucose supply rate, generation of surplus reduction equivalents resulted in simultaneous ethanol formation. On the other hand, at a low glucose supply rate, additional reduction equivalents were generated by simultaneous ethanol consumption. A significantly lower xylitol formation rate was observed.
摘要
在表达木糖还原酶基因（xyl1）的重组酿酒酵母菌株将木糖转化为木糖醇的厌氧条件下，葡萄糖用作共底物。葡萄糖主要通过糖酵解代谢，最终产物为二氧化碳、乙酸和乙醇，并在甘油醛-3-磷酸脱氢酶和乙醛脱氢酶反应中产生还原当量。在高葡萄糖供应速率下，过量还原当量的产生导致同时形成乙醇。另一方面，在低葡萄糖供应速率下，通过同时消耗乙醇产生额外的还原当量。观察到木糖醇形成速率明显较低。
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