用 C 核磁共振波谱研究毕赤酵母 CBS 6054 中的乙醇再摄取和乙醇耐受性。
Ethanol Reassimilation and Ethanol Tolerance in Pichia stipitis CBS 6054 as Studied by C Nuclear Magnetic Resonance Spectroscopy.
机构信息
Department of Applied Microbiology, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden, and Department of Biochemistry and Department of Chemistry, University of Odense, Campusvej 55, DK-5230 Odense M, Denmark.
出版信息
Appl Environ Microbiol. 1992 Aug;58(8):2552-8. doi: 10.1128/aem.58.8.2552-2558.1992.
Abstract
Ethanol reassimilation in Pichia stipitis CBS 6054 was studied by using continuous cultures, and the oxidation of [1-C]ethanol was monitored by in vivo and in vitro C nuclear magnetic resonance spectroscopy. Acetate was formed when ethanol was reassimilated. The ATP/ADP ratio and the carbon dioxide production decreased, whereas the malate dehydrogenase activity increased, in ethanol-reassimilating cells. The results are discussed in terms of the low ethanol tolerance in P. stipitis compared with that in Saccharomyces cerevisiae (S. W. Brown, S. G. Oliver, D. E. F. Harrison, and R. C. Righelato, Eur. J. Appl. Microbiol. Biotechnol. 11:151-155, 1981).
摘要
研究了利用连续培养，在毕赤酵母 CBS 6054 中重新同化乙醇，并用体内和体外 C 核磁共振光谱监测[1-C]乙醇的氧化。当乙醇被重新同化时，形成了乙酸盐。在乙醇再同化细胞中，ATP/ADP 比值和二氧化碳产生减少，而苹果酸脱氢酶活性增加。根据毕赤酵母与酿酒酵母相比的低乙醇耐受性（S. W. Brown、S. G. Oliver、D. E. F. Harrison 和 R. C. Righelato，Eur. J. Appl. Microbiol. Biotechnol. 11:151-155, 1981）对这些结果进行了讨论。
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