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Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.酿酒酵母在不同碳源上有氧生长期间酶活性的变化。
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Effect of Oxygenation on Xylose Fermentation by Pichia stipitis.供氧对毕赤酵母木糖发酵的影响。
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Concurrent Production and Consumption of Ethanol by Cultures of Pachysolen tannophilus Growing on d-Xylose.塔宾曲霉培养物利用 d-木糖同时生产和消耗乙醇。
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Effect of ethanol and acetaldehyde on membrane-bound enzymes in rat brain.
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Multiple forms of alcohol dehydrogenase in Saccharomyces cerevisiae. I. Physiological control of ADH-2 and properties of ADH-2 and ADH-4.酿酒酵母中多种形式的乙醇脱氢酶。I. ADH-2的生理调控以及ADH-2和ADH-4的特性
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Primary structure requirements for correct sorting of the yeast mitochondrial protein ADH III to the yeast mitochondrial matrix space.酵母线粒体蛋白ADH III正确分选至酵母线粒体基质空间的一级结构要求。
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31P nuclear magnetic resonance study of the effect of azide on xylose fermentation by Candida tropicalis.叠氮化物对热带假丝酵母木糖发酵影响的31P核磁共振研究
Appl Environ Microbiol. 1989 Aug;55(8):1974-80. doi: 10.1128/aem.55.8.1974-1980.1989.
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Effects of ethanol on Saccharomyces cerevisiae as monitored by in vivo 31P and 13C nuclear magnetic resonance.通过体内31P和13C核磁共振监测乙醇对酿酒酵母的影响。
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用 C 核磁共振波谱研究毕赤酵母 CBS 6054 中的乙醇再摄取和乙醇耐受性。

Ethanol Reassimilation and Ethanol Tolerance in Pichia stipitis CBS 6054 as Studied by C Nuclear Magnetic Resonance Spectroscopy.

机构信息

Department of Applied Microbiology, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden, and Department of Biochemistry and Department of Chemistry, University of Odense, Campusvej 55, DK-5230 Odense M, Denmark.

出版信息

Appl Environ Microbiol. 1992 Aug;58(8):2552-8. doi: 10.1128/aem.58.8.2552-2558.1992.

DOI:10.1128/aem.58.8.2552-2558.1992
PMID:16348754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC195820/
Abstract

Ethanol reassimilation in Pichia stipitis CBS 6054 was studied by using continuous cultures, and the oxidation of [1-C]ethanol was monitored by in vivo and in vitro C nuclear magnetic resonance spectroscopy. Acetate was formed when ethanol was reassimilated. The ATP/ADP ratio and the carbon dioxide production decreased, whereas the malate dehydrogenase activity increased, in ethanol-reassimilating cells. The results are discussed in terms of the low ethanol tolerance in P. stipitis compared with that in Saccharomyces cerevisiae (S. W. Brown, S. G. Oliver, D. E. F. Harrison, and R. C. Righelato, Eur. J. Appl. Microbiol. Biotechnol. 11:151-155, 1981).

摘要

研究了利用连续培养，在毕赤酵母 CBS 6054 中重新同化乙醇，并用体内和体外 C 核磁共振光谱监测[1-C]乙醇的氧化。当乙醇被重新同化时，形成了乙酸盐。在乙醇再同化细胞中，ATP/ADP 比值和二氧化碳产生减少，而苹果酸脱氢酶活性增加。根据毕赤酵母与酿酒酵母相比的低乙醇耐受性（S. W. Brown、S. G. Oliver、D. E. F. Harrison 和 R. C. Righelato，Eur. J. Appl. Microbiol. Biotechnol. 11:151-155, 1981）对这些结果进行了讨论。