表达异源XYL1基因的酿酒酵母甘油-3-磷酸脱氢酶缺陷型突变体。
A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene.
作者信息
Lidén G, Walfridsson M, Ansell R, Anderlund M, Adler L, Hahn-Hägerdal B
机构信息
Department of Chemical Reaction Engineering, Chalmers University of Technology, Göteborg, Sweden.
出版信息
Appl Environ Microbiol. 1996 Oct;62(10):3894-6. doi: 10.1128/aem.62.10.3894-3896.1996.
Abstract
The gene XYL1, encoding a xylose reductase, from Pichia stipitis was transformed into a mutant of Saccharomyces cerevisiae incapable of glycerol production because of deletion of the genes GPD1 and GPD2. The transformed strain was capable of anaerobic glucose conversion in the presence of added xylose, indicating that the xylose reductase reaction can fulfill the role of the glycerol-3-phosphate dehydrogenase reaction as a redox sink. The specific xylitol production rate obtained was 0.38 g g-1 h-1.
摘要
将树干毕赤酵母中编码木糖还原酶的XYL1基因转化到酿酒酵母的一个突变体中，该突变体由于GPD1和GPD2基因缺失而无法产生甘油。转化后的菌株能够在添加木糖的情况下进行厌氧葡萄糖转化，这表明木糖还原酶反应可以作为氧化还原汇发挥甘油-3-磷酸脱氢酶反应的作用。得到的木糖醇比生产速率为0.38 g g-1 h-1。
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Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the xyl1 gene.在表达木糖异构酶基因（xyl1）的重组酿酒酵母中，以葡萄糖作为共底物进行厌氧木糖转化过程中的木糖醇形成及还原当量生成
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