将在其裂解位点发生突变的仙台病毒F基因cDNA和HN基因cDNA转染到COS细胞中可诱导细胞融合。
Transfection of Sendai virus F gene cDNA with mutations at its cleavage site and HN gene cDNA into COS cells induces cell fusion.
作者信息
Taira H, Sato T, Segawa H, Chiba M, Katsumata T, Iwasaki K
机构信息
Department of Bioscience and Technology, Faculty of Agriculture, Iwate University, Morioka, Japan.
出版信息
Arch Virol. 1995;140(1):187-94. doi: 10.1007/BF01309734.
PMID:7646344
Abstract
In contrast to the wild type Sendai virus fusion protein (F), a mutated F to possess a cleavage site similar to that of virulent Newcastle disease virus F, could be cleaved by proteases present in COS cells. When mutated F and hemagglutinin-neuraminidase (HN) were coexpressed at the cell surface, syncytium formation was observed.
摘要
与野生型仙台病毒融合蛋白（F）相比，一种经过突变、具有与强毒性新城疫病毒F相似切割位点的F蛋白，可被COS细胞中存在的蛋白酶切割。当突变的F蛋白和血凝素-神经氨酸酶（HN）在细胞表面共表达时，可观察到合胞体形成。
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