孕酮对体外培养的小鼠肾上腺素能神经末梢去甲肾上腺素释放的影响。

Effect of progesterone on norepinephrine release from mouse adrenergic terminals in vitro.

Padilla L, Asencio H, Fernandez W, Cruz M A, Neumann V, Reinicke K, Rudolph M I

Departamento de Ciencias Fisológicas, Facultad de Ciencias Biológicas y de Recursos Naturales, Universidad de Concepción, Chile.

Gen Pharmacol. 1991;22(4):647-50. doi: 10.1016/0306-3623(91)90071-d.

Abstract

The in vitro effect of progesterone on norepinephrine (NE) release and contractile activity was analyzed in uterine horns from estrogen-primed and progesterone-primed mice. 2. Progesterone (6-10 nmol/ml) evoked the release of [3H]NE above basal levels from uterine horns in both experimental conditions, the effect of progesterone on estrogen-primed being more important than on progesterone-primed mice uterus. 3. Progesterone also increased electrically evoked [3H]NE release in estrogen-primed uterine tissue, nevertheless no effect was observed in progesterone-primed ones. 4. Progesterone (0.6-10 nmol/ml) inhibited uterine horn isometric contractions only in estradiol-primed mice. This effect was partially blocked in uterine horns from reserpine-treated mice and when propanolol (1 microM) was added to the preparation of estradiol-primed mice uterus.

摘要

在雌激素预处理和孕酮预处理的小鼠子宫角中分析了孕酮对去甲肾上腺素（NE）释放和收缩活性的体外作用。2. 在两种实验条件下，孕酮（6 - 10 nmol/ml）均使子宫角中[3H]NE的释放高于基础水平，孕酮对雌激素预处理小鼠子宫的作用比对孕酮预处理小鼠子宫的作用更显著。3. 孕酮还增加了雌激素预处理子宫组织中电诱发的[3H]NE释放，然而在孕酮预处理的子宫组织中未观察到这种作用。4. 孕酮（0.6 - 10 nmol/ml）仅在雌二醇预处理的小鼠中抑制子宫角等长收缩。在利血平处理的小鼠子宫角中以及向雌二醇预处理的小鼠子宫制备物中加入普萘洛尔（1 microM）时，这种作用被部分阻断。