Kubicka-Muranyi M, Griem P, Lübben B, Rottmann N, Lührmann R, Gleichmann E
Division of Immunology, University Düsseldorf, Germany.
Int Arch Allergy Immunol. 1995 Sep;108(1):1-10. doi: 10.1159/000237110.
HgCl2 treatment of B10.S mice induces IgG autoantibodies to fibrillarin, a component of small nuclear ribonucleoprotein particles, and histone. Here, we demonstrate the activation by HgCl2 of autoreactive T cells specific for these nuclear proteins. Of nine CD4+ T cell hybridoma clones obtained from HgCl2-treated B10.S mice, one clone reacted to histone Hl and eight clones to fibrillarin. One of the fibrillarin-specific clones only recognized fibrillarin pretreated with HgCl2 (Hg2+ fibrillarin), suggesting that Hg2+ can induce the presentation of a novel fibrillarin epitope. Four fibrillarin-specific hybridoma clones studied for cytokine production were shown to produce interleukin (IL)-2, and three of them also produced IL-4. For stimulation of fibrillarin-specific T cell hybridomas, exogenous murine fibrillarin had to be added when antigen-presenting cells (APCs) came from untreated mice, but not when the APCs were obtained from HgCl2-treated animals. Apparently, HgCl2 treatment induces the presentation by APCs of a novel Hg2+ fibrillarin epitope and up-regulates the presentation of unaltered fibrillarin epitopes, thus activating 'Hg(2+)-specific' as well as autoreactive CD4+ T cells.
用氯化汞处理B10.S小鼠可诱导产生针对小核糖核蛋白颗粒成分原纤维蛋白和组蛋白的IgG自身抗体。在此，我们证明了氯化汞可激活针对这些核蛋白的自身反应性T细胞。从经氯化汞处理的B10.S小鼠获得的9个CD4+ T细胞杂交瘤克隆中，一个克隆对组蛋白H1有反应，8个克隆对原纤维蛋白有反应。其中一个原纤维蛋白特异性克隆仅识别经氯化汞预处理的原纤维蛋白（Hg2+原纤维蛋白），这表明Hg2+可诱导呈现一种新的原纤维蛋白表位。对4个研究细胞因子产生的原纤维蛋白特异性杂交瘤克隆进行检测，结果显示它们可产生白细胞介素（IL）-2，其中3个还产生IL-4。为刺激原纤维蛋白特异性T细胞杂交瘤，当抗原呈递细胞（APC）来自未处理的小鼠时，必须添加外源性小鼠原纤维蛋白，但当APC取自经氯化汞处理的动物时则无需添加。显然，氯化汞处理可诱导APC呈现新的Hg2+原纤维蛋白表位，并上调未改变的原纤维蛋白表位的呈递，从而激活“Hg(2+)特异性”以及自身反应性CD4+ T细胞。
