Chin K, Morise K, Kanayama K, Nagura H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
J Gastroenterol. 1995 Jun;30(3):330-7. doi: 10.1007/BF02347508.
The localization and distribution of gamma delta T cell receptor (TCR)-positive cells (gamma delta T cells) in hepatocellular carcinoma capsules was investigated immunohistochemically at both light and electron microscopic levels. Most of the mononuclear cells infiltrating the tumor capsules were CD3-positive. Together with gamma delta T cells, they were significantly increased in the tumor capsules compared to amounts in the fibrous septa in non-cancerous cirrhotic areas of the same liver, and compared to amounts in the liver of patients with cirrhosis. Phenotypic characterization by the two-color double-staining technique showed that CD8/gamma delta cells were significantly increased in the tumor capsule, and that more than one-third of gamma delta TCR-positive cells also expressed the CD56 antigen. Morphological observation revealed that large gamma delta T cells were increased in number in the tumor capsule and that the cytoplasm of these cells contained multivesicular bodies and dense granules. These morphological features were similar to those of large granular lymphocytes, and most of the gamma delta T cells were also positive for BB3. This suggests that extrathymic maturation of gamma delta T cells occurs in the tumor capsule, and that these gamma delta T cells may have a cytolytic effect on tumor cells, as shown in large granular lymphocytes; further, the results suggest that these cells may play a role in the defense against tumor expansion.
采用免疫组织化学方法，在光镜和电镜水平上研究了γδT细胞受体（TCR）阳性细胞（γδT细胞）在肝细胞癌包膜中的定位和分布。浸润肿瘤包膜的大多数单核细胞为CD3阳性。与γδT细胞一起，与同一肝脏非癌性肝硬化区域纤维间隔中的数量相比，以及与肝硬化患者肝脏中的数量相比，它们在肿瘤包膜中显著增加。通过双色双重染色技术进行的表型特征分析表明，肿瘤包膜中CD8/γδ细胞显著增加，并且超过三分之一的γδTCR阳性细胞也表达CD56抗原。形态学观察显示，肿瘤包膜中大型γδT细胞数量增加，并且这些细胞的细胞质中含有多囊泡体和致密颗粒。这些形态学特征与大颗粒淋巴细胞相似，并且大多数γδT细胞对BB3也呈阳性。这表明γδT细胞在肿瘤包膜中发生胸腺外成熟，并且这些γδT细胞可能对肿瘤细胞具有细胞溶解作用，如大颗粒淋巴细胞所示；此外，结果表明这些细胞可能在抵抗肿瘤扩展中发挥作用。
