Kanayama K, Morise K, Nagura H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Am J Gastroenterol. 1992 Aug;87(8):1018-22.
The distribution and phenotypic characterization of T cell receptor (TCR) gamma delta cells in human liver tissue was investigated immunohistochemically at light and electron microscopic levels. In chronic liver disease, there was a significant increase in the number of TCR gamma delta cells and in the percentage of TCR gamma delta cells to CD3+ cells in the portal areas and hepatic sinusoids. Hepatic TCR gamma delta cells were classified as small or large gamma delta cells. Large gamma delta cells were increased in chronic liver disease, whereas both small and large gamma delta cells were increased in the portal areas and hepatic sinusoids in liver cirrhosis. The increased TCR gamma delta cells were of the BB3+ (peripheral) type, indicating that TCR gamma delta cells in the liver were of the same lineage as those in the peripheral blood. In addition, the majority of the TCR gamma delta cells in the portal areas of liver cirrhosis patients were CD4- and CD8- (double negative). Immunoelectron microscopy showed that the large gamma delta cells were lymphoblastoid and contained multivesicles. The present study clearly demonstrated that there are two types of TCR gamma delta cells, and that these cells were significantly increased in the livers of patients with chronic liver disease. This suggests that they may be involved in regulation of the immune response and hepatocellular damage in chronic liver disease.
在光学和电子显微镜水平上，采用免疫组织化学方法研究了人类肝脏组织中T细胞受体（TCR）γδ细胞的分布及表型特征。在慢性肝病中，门管区和肝血窦内TCRγδ细胞的数量以及TCRγδ细胞占CD3+细胞的百分比显著增加。肝内TCRγδ细胞分为小γδ细胞或大γδ细胞。大γδ细胞在慢性肝病中增多，而在肝硬化患者的门管区和肝血窦内，小γδ细胞和大γδ细胞均增多。增多的TCRγδ细胞属于BB3+（外周）型，表明肝脏中的TCRγδ细胞与外周血中的TCRγδ细胞属于同一谱系。此外，肝硬化患者门管区的大多数TCRγδ细胞为CD4-和CD8-（双阴性）。免疫电子显微镜显示，大γδ细胞呈淋巴母细胞样，含有多个小泡。本研究清楚地表明，存在两种类型的TCRγδ细胞，且这些细胞在慢性肝病患者的肝脏中显著增多。这表明它们可能参与慢性肝病中免疫反应的调节和肝细胞损伤。
