Koning F, Stingl G, Yokoyama W M, Yamada H, Maloy W L, Tschachler E, Shevach E M, Coligan J E
Science. 1987 May 15;236(4803):834-7. doi: 10.1126/science.2883729.
The murine epidermis contains a subpopulation of bone marrow-derived lymphocytes that have a dendritic morphology and that express Thy-1 and T3 cell-surface antigens but not other markers (L3T4 or Lyt-2) characteristic of mature peripheral T lymphocytes. An alternative type of T cell receptor was earlier identified on a subpopulation of murine thymocytes with a similar phenotype (T3+, L3T4-, Lyt-2-), but not on peripheral murine T lymphocytes. Two independently derived Thy-1+, L3T4-, and Lyt-2- dendritic cell lines of epidermal origin that express a T3-associated disulfide-linked heterodimer composed of a 34-kilodalton gamma-chain and 46-kilodalton partner (the delta chain) have now been identified. Analysis of N-linked glycosylation revealed that this receptor is similar to that detected on thymocytes. These results demonstrate that Thy-1+ dendritic epidermal cell lines can express gamma delta T cell receptors in vitro and suggest that Thy-1+ dendritic epidermal cells express such receptors in vivo. The localization of these gamma delta T cell receptor-expressing cells in the epidermis may be of importance for understanding the function of these receptors.
小鼠表皮含有一群源自骨髓的淋巴细胞，这些细胞具有树突状形态，表达Thy-1和T3细胞表面抗原，但不表达成熟外周T淋巴细胞特有的其他标志物（L3T4或Lyt-2）。早期在一群具有相似表型（T3 +、L3T4 -、Lyt-2 -）的小鼠胸腺细胞上鉴定出了另一种类型的T细胞受体，但在外周小鼠T淋巴细胞上未鉴定出。现已鉴定出两种独立衍生的源自表皮的Thy-1 +、L3T4 -和Lyt-2 -树突状细胞系，它们表达一种与T3相关的由34千道尔顿的γ链和46千道尔顿的伴侣（δ链）组成的二硫键连接的异二聚体。对N-连接糖基化的分析表明，这种受体与在胸腺细胞上检测到的受体相似。这些结果表明，Thy-1 +树突状表皮细胞系在体外可表达γδ T细胞受体，并提示Thy-1 +树突状表皮细胞在体内表达此类受体。这些表达γδ T细胞受体的细胞在表皮中的定位可能对于理解这些受体的功能很重要。
