Akizuki S, Magara T, Tanaka T
Department of Laboratory Medicine, Jikei University, Daisan Hospital, Tokyo.
Nihon Rinsho Meneki Gakkai Kaishi. 1998 Jun;21(3):108-17. doi: 10.2177/jsci.21.108.
gamma delta T lymphocytes, which are CD3+ lymphocytes that express gamma delta chains of the T-cell antigen receptor (TCR) on their surface, are functionally distinct from alpha beta T lymphocytes, which express alpha beta chains of the TCR. gamma delta T lymphocytes are thought to differentiate in mouse hepatic sinusoids, to play a role in antitumor action, and to act as natural killer cells. The purpose of this study was to examine whether gamma delta T lymphocytes in the peripheral blood are suppressed when hepatic sinusoids are damaged during transcatheter arterial embolization (TAE). The numbers of alpha beta T lymphocytes and gamma delta T lymphocytes in the peripheral blood were examined with monoclonal antibodies and flow cytometry before and after TAE in 32 patients (from 46 to 78 years of age) with liver cirrhosis and hepatocellular carcinoma. The number of alpha beta T lymphocytes before and after TAE was unchanged. However, the number of gamma delta T lymphocytes and the ratio of gamma delta T lymphocytes to CD3+ lymphocytes were significantly decreased for 3 weeks after TAE treatment. This decrease suggests that TAE suppresses the supply of gamma delta T lymphocytes to the peripheral blood. In addition, TAE may weaken a patient's antitumor immunity, because gamma delta T lymphocytes that have antitumor activity decrease after TAE.
γδ T淋巴细胞是一种CD3 +淋巴细胞，其表面表达T细胞抗原受体（TCR）的γδ链，在功能上与表达TCR的αβ链的αβ T淋巴细胞不同。γδ T淋巴细胞被认为在小鼠肝血窦中分化，在抗肿瘤作用中发挥作用，并作为自然杀伤细胞。本研究的目的是探讨经导管动脉栓塞术（TAE）期间肝血窦受损时，外周血中的γδ T淋巴细胞是否受到抑制。采用单克隆抗体和流式细胞术检测了32例（46至78岁）肝硬化合并肝细胞癌患者TAE前后外周血中αβ T淋巴细胞和γδ T淋巴细胞的数量。TAE前后αβ T淋巴细胞的数量没有变化。然而，TAE治疗后3周，γδ T淋巴细胞的数量以及γδ T淋巴细胞与CD3 +淋巴细胞的比例显著降低。这种降低表明TAE抑制了γδ T淋巴细胞向外周血的供应。此外，TAE可能会削弱患者的抗肿瘤免疫力，因为具有抗肿瘤活性的γδ T淋巴细胞在TAE后减少。
