Ryu J S, Lloyd D
Department of Parasitology, College of Medicine, Hanyang University, Seoul, South Korea.
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):183-7. doi: 10.1111/j.1574-6968.1995.tb07717.x.
We have investigated the action of sodium nitrite and other nitrosyl complexes, such as sodium nitroprusside and Roussin's black salt, on the growth of metronidazole-sensitive and resistant strains of Trichomonas vaginalis and their hydrogenosomal enzymes. All three chemicals inhibited the growth of T. vaginalis: sodium nitrite at 8 mM, sodium nitroprusside at 1.2 mM and Roussin's black salt at 0.2 mM. Metronidazole-sensitive (KT9) and resistant (CDC85) isolates showed similar cytotoxicity against these molecules. Specific activities of pyruvate:ferredoxin oxidoreductase and hydrogenase and oxygen uptake rates were decreased in the T. vaginalis isolate treated with sodium nitrite and sodium nitroprusside. However, Roussin's black salt increased the specific activity of pyruvate:ferredoxin oxidoreductase or hydrogenase in CDC85 or KT9 cells and increased the oxygen uptake rate in the KT9 isolate.
我们研究了亚硝酸钠以及其他亚硝酰基配合物，如硝普钠和鲁辛黑盐，对甲硝唑敏感和耐药的阴道毛滴虫菌株生长及其氢化酶体酶的作用。这三种化学物质均抑制阴道毛滴虫的生长：亚硝酸钠浓度为8 mM，硝普钠浓度为1.2 mM，鲁辛黑盐浓度为0.2 mM。甲硝唑敏感菌株（KT9）和耐药菌株（CDC85）对这些分子表现出相似的细胞毒性。在用亚硝酸钠和硝普钠处理的阴道毛滴虫菌株中，丙酮酸：铁氧化还原蛋白氧化还原酶和氢化酶的比活性以及氧气摄取率均降低。然而，鲁辛黑盐提高了CDC85或KT9细胞中丙酮酸：铁氧化还原蛋白氧化还原酶或氢化酶的比活性，并提高了KT9菌株的氧气摄取率。
