来自化脓性链球菌的NAD(+) - 糖水解酶具有环化ADP - 核糖形成活性。
NAD(+)-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity.
作者信息
Karasawa T, Takasawa S, Yamakawa K, Yonekura H, Okamoto H, Nakamura S
机构信息
Department of Bacteriology, School of Medicine, Kanazawa University, Japan.
出版信息
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):201-4. doi: 10.1111/j.1574-6968.1995.tb07720.x.
NAD(+)-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.
通过在CM Sepharose CL - 6B、Sephacryl S - 200 HR和羟基磷灰石上连续色谱法纯化了化脓性链球菌的NAD(+) - 糖水解酶。纯化后的酶具有环状ADP - 核糖（cADPR）的合成和水解活性，cADPR是新发现的用于钙离子动员的第二信使，同时还具有NAD+中核糖 - 烟酰胺键的裂解活性。
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