Qiu C X, Ferguson M W
Cells, Immunology and Development Division, School of Biological Sciences, University of Manchester, UK.
J Anat. 1995 Feb;186 ( Pt 1)(Pt 1):17-29.
The distribution of the 2 homodimer isoforms of platelet-derived growth factor (PDGF)-AA and -BB, and their receptors (PDGF alpha-receptor and PDGF beta-receptor) were mapped immunocytochemically during the development of the embryonic mouse secondary palate, from embryonic day (E) 12 to E15. The staining patterns of PDGF-AA/alpha-receptor and PDGF-BB/beta-receptor are different. Generally, the former is present and the latter is sparse/absent during the period of palate development. The localisations of PDGF-AA and alpha-receptor are more intensive in the epithelia than in the mesenchyme, and change temporally during palatal shelf elevation, midline epithelial seam formation and disruption. Before the palatal shelf has elevated (E13), PDGF alpha-receptor is present in the palatal mesenchyme, nasal and medial edge epithelia (MEE), whilst PDGF-AA stains lightly in the palatal epithelia. During palatal midline epithelial seam formation and following its degeneration throughout E14, PDGF-AA and PDGF alpha-receptor were intensively colocalised in the nasal and midline seam epithelia. These findings indicate that the interaction between PDGF-AA and its receptor may participate in palate development, particularly in regulating palatal midline epithelial seam formation and degeneration.
在胚胎小鼠次生腭从胚胎第（E）12天到E15天的发育过程中，通过免疫细胞化学方法绘制了血小板衍生生长因子（PDGF）-AA和-BB的两种同二聚体亚型及其受体（PDGFα受体和PDGFβ受体）的分布图。PDGF-AA/α受体和PDGF-BB/β受体的染色模式不同。一般来说，在腭发育期间，前者存在而后者稀疏/不存在。PDGF-AA和α受体在上皮中的定位比在间充质中更密集，并且在腭突抬高、中线上皮缝形成和破坏过程中随时间变化。在腭突抬高之前（E13），PDGFα受体存在于腭间充质、鼻和内侧边缘上皮（MEE）中，而PDGF-AA在腭上皮中染色较轻。在腭中线上皮缝形成期间以及在整个E14期间其退化之后，PDGF-AA和PDGFα受体在鼻和中线缝上皮中强烈共定位。这些发现表明，PDGF-AA与其受体之间的相互作用可能参与腭的发育，特别是在调节腭中线上皮缝的形成和退化方面。
