Tanaka Y, Sugoh M, Ji W, Iwabuchi J, Yoshida H, Omura S
Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
J Antibiot (Tokyo). 1995 Jul;48(7):720-4. doi: 10.7164/antibiotics.48.720.
A new screening method for inhibitors of cellulose biosynthesis is described. This method utilized three microbial strains; a cellulose-containing fungus Phytophthora, and a cellulose non-containing fungus Candida, and a bacterial strain of Acetobacter, a cellulose-producing acetic acid bacterium. The primary screen examined microbial cultures for selective growth inhibition against Phytophthora with no inhibition against Candida. The secondary screen tested for herbicidal activity. Thirdly, the active cultures were examined for their inhibition of cellulose biosynthesis by an Acetobacter strain. A screening trial with this new method led to the discovery of two microbial metabolites named phthoxazolin A and phthoramycin as new inhibitors of cellulose biosynthesis with herbicidal activity.
本文描述了一种用于筛选纤维素生物合成抑制剂的新方法。该方法使用了三种微生物菌株：一种含纤维素的真菌疫霉属、一种不含纤维素的真菌假丝酵母属，以及一种产纤维素的醋酸杆菌属细菌菌株。初步筛选检测微生物培养物对疫霉属的选择性生长抑制，而对假丝酵母属无抑制作用。二次筛选检测除草活性。第三，检测活性培养物对醋酸杆菌属菌株纤维素生物合成的抑制作用。用这种新方法进行的筛选试验发现了两种微生物代谢产物，即邻苯恶唑啉A和邻苯霉素，它们是具有除草活性的新型纤维素生物合成抑制剂。
