通过一种新型内部对照PCR检测法定量人类免疫缺陷病毒1型DNA和RNA。
Quantitation of human immunodeficiency virus type 1 DNA and RNA by a novel internally controlled PCR assay.
作者信息
Gupta P, Ding M, Cottrill M, Rinaldo C, Kingsley L, Wolinsky S, Mellors J
机构信息
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, PA 15261, USA.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1670-3. doi: 10.1128/jcm.33.6.1670-1673.1995.
Abstract
A novel internally controlled PCR (ICPCR) assay was developed to accurately quantitate human immunodeficiency virus type 1 (HIV-1) DNA and RNA in peripheral blood mononuclear cells and plasma. The ICPCR assay was sensitive and reproducible within a linear range of amplification of 10(0) to 10(3) copies for HIV-1 DNA and 10(1) to 10(4) copies for HIV-1 RNA. The assay detected HIV-1 RNA in plasma and peripheral blood mononuclear cells from all HIV-1 subjects regardless of disease stage. ICPCR was compared with a branched-DNA signal amplification assay for subjects beginning antiretroviral therapy. The reductions in plasma HIV-1 RNA in response to therapy were comparable with the two assays. The ICPCR assay should be useful in monitoring HIV-1 RNA levels both in natural history studies and in clinical trials of antiretroviral agents.
摘要
开发了一种新型的内控PCR（ICPCR）检测方法，用于准确定量外周血单核细胞和血浆中的1型人类免疫缺陷病毒（HIV-1）DNA和RNA。ICPCR检测方法在HIV-1 DNA扩增的线性范围内（10(0)至10(3)拷贝）以及HIV-1 RNA扩增的线性范围内（10(1)至10(4)拷贝）具有敏感性和可重复性。该检测方法能检测出所有HIV-1感染者血浆和外周血单核细胞中的HIV-1 RNA，无论疾病处于何阶段。将ICPCR与用于开始抗逆转录病毒治疗的受试者的分支DNA信号扩增检测方法进行了比较。两种检测方法检测到的血浆中HIV-1 RNA因治疗而降低的情况相当。ICPCR检测方法在自然史研究和抗逆转录病毒药物临床试验中监测HIV-1 RNA水平时应会很有用。
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本文引用的文献
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Human immunodeficiency virus type 1 (HIV-1) infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men.在诊断性免疫印迹法检测前，人类免疫缺陷病毒1型（HIV-1）感染的中位时间为18个月。来自一组同性恋男性的证据。
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Detection and quantification of human immunodeficiency virus RNA in patient serum by use of the polymerase chain reaction.利用聚合酶链反应检测和定量患者血清中的人类免疫缺陷病毒RNA。
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